One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography

被引：6

作者：

Liu, Yongling ^{[1
,2
]}

Wang, Ping ^{[1
,2
]}

Chen, Tao ^{[1
]}

Jia, Jing ^{[1
]}

Sun, Jing ^{[1
]}

You, Jinmao ^{[1
]}

Li, Yulin ^{[1
]}

机构：

[1] Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2014年 / 49卷 / 07期

关键词：

Halenia elliptica; high-speed counter-current chromatography; xanthone glycosides; SEPARATION; GLUCOSIDES;

D O I：

10.1080/01496395.2013.872659

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

High-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four xanthone glycosides from Halenia elliptica, a plant widely used in traditional Tibetan medicine. The introduction of HSCCC greatly improved the efficiency of compounds preparation from Halenia elliptica. The following were obtained from 100 mg of crude sample in one-step separation: 2.5 mg of 1-O-primeverosyl-2,3,4,5,7-pentamethoxyxanthone, 7.0 mg of 1-O-primeverosyl-2,3,4,7- tetramethoxyxanthone, 10.0 mg of 1-O-primeverosyl-2,3,5-trimethoxyxanthone (demethyoxyhaleniaside), and 8.5 mg of 1-O-primeverosyl-2,3,4,5-tetramethoxyxanthone. HPLC analysis showed that each target compound had a purity of over 98%, and UV, H-1 NMR, and C-13 NMR data confirmed the component chemical structures.

引用

页码：1119 / 1124

页数：6

共 16 条

[1] 2 XANTHONE GLUCOSIDES FROM HALENIA-ELLIPTICA [J].