RSV replication is attenuated by counteracting expression of the suppressor of cytokine signaling (SOCS) molecules

被引：48

作者：

Hashimoto, Koichi ^{[1
,2
]}

Ishibashi, Kei ^{[2
,3
]}

Ishioka, Ken ^{[2
]}

Zhao, Dongchi ^{[2
]}

Sato, Masatoki ^{[1
]}

Ohara, Shinichiro ^{[1
]}

Abe, Yusaku ^{[1
]}

Kawasaki, Yukihiko ^{[1
]}

Sato, Yuka ^{[2
]}

Yokota, Shin-ichi ^{[4
]}

Fujii, Nobuhiro ^{[4
]}

Peebles, Ray Stokes, Jr. ^{[5
]}

Hosoya, Mitsuaki ^{[1
]}

Suzutani, Tatsuo ^{[2
]}

机构：

[1] Fukushima Med Univ, Sch Med, Dept Pediat, Fukushima 9601295, Japan

[2] Fukushima Med Univ, Sch Med, Dept Microbiol, Fukushima 9601295, Japan

[3] Fukushima Med Univ, Sch Med, Dept Urol, Fukushima 9601295, Japan

[4] Sapporo Med Univ, Sch Med, Dept Microbiol, Sapporo, Hokkaido 0608556, Japan

[5] Vanderbilt Univ, Med Ctr, Ctr Lung Res, MCN T1217, Nashville, TN 37232 USA

来源：

VIROLOGY | 2009年 / 391卷 / 02期

基金：

日本学术振兴会;

关键词：

RSV; IFN; SOCS; siRNA; JAK/STAT; RESPIRATORY-SYNCYTIAL-VIRUS; NEGATIVE REGULATION; INTERFERON-ALPHA; EPITHELIAL-CELLS; INNATE IMMUNITY; PROTEINS; INHIBITION; INDUCTION; INFECTION; ALLERGY;

D O I：

10.1016/j.virol.2009.06.026

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human RSV causes an annual epidemic of respiratory tract illness in infants and in elderly. Mechanisms by which RSV antagonizes IFN-mediated antiviral responses include inhibition of type I IFN mRNA transcription and blocking signal transduction of JAK/STAT family members. The suppressor of cytokines signaling (SOCS) gene family utilizes a feedback loop to inhibit cytokine responses and block the activation of the JAK/STAT signaling pathway. To evaluate the potential of SOCS molecules to subvert the innate immune response to RSV infection, eight SOCS family genes were examined. RSV infection up-regulated SOCS1, SOCS3, and CIS mRNA expression in HEp-2 cells, Suppression of SOCS 1, SOCS3 and CIS by short interfering ribonucleic acid (siRNA) inhibited viral replication. Furthermore, inhibition of SOCS1, SOCS3, or CIS activated type I IFN signaling by inducing STAT1/2 phosphorylation. These results suggest that RSV infection escapes the innate antiviral response by inducing SOCS1, SOCS3 or CIS expression in epithelial cells. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：162 / 170

页数：9

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