In situ detection of cellular and abnormal isoforms of prion protein in brains of cattle with bovine spongiform encephalopathy and sheep with scrapie by use of a histoblot technique

被引：4

作者：

Kimura, KM ^{[1
]}

Yokoyama, T

Haritani, M

Narita, M

Belleby, P

Smith, J

Spencer, YI

机构：

[1] Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan

[2] Natl Inst Anim Hlth, Shichinohe Res Unit, Kamikita, Aomori 0392586, Japan

[3] Vet Lab Agcy, Weybridge KT15 3NB, Surrey, England

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2002年 / 14卷 / 03期

关键词：

D O I：

10.1177/104063870201400313

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and Sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrPC) and abnormal (PrPSc,) isoforms of the prion protein. In BSE- or scrapie-affected brains, the PrPC signal decreased, especially in those areas where the PrPSc signal was detected.

引用

页码：255 / 257

页数：3

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