Significance of hyphae formation in virulence of Candida tropicalis and transcriptomic analysis of hyphal cells

被引：29

作者：

Jiang, Cen ^{[1
]}

Li, Zhen ^{[1
]}

Zhang, Lihua ^{[1
]}

Tian, Yuan ^{[1
]}

Dong, Danfeng ^{[1
]}

Peng, Yibing ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Lab Med, Sch Med, Shanghai, Peoples R China

来源：

MICROBIOLOGICAL RESEARCH | 2016年 / 192卷

基金：

中国国家自然科学基金;

关键词：

Hyphae; Virulence; Candida tropicalis; RNA-seq; HUMAN FUNGAL PATHOGEN; SUPEROXIDE DISMUTASES; OXIDATIVE STRESS; ALBICANS; MORPHOGENESIS; PHOSPHOLIPASE; INFECTIONS; PROTEINASE; MECHANISMS; RESISTANCE;

D O I：

10.1016/j.micres.2016.06.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recently, the proportion of Candida tropicalis in clinical isolates has significantly increased. Some C tropicalis strains colonize the skin or mucosal surfaces as commensals; others trigger invasive infection. To date, the pathogenicity of C. tropicalis has not been thoroughly researched. This study reports several virulence factors, including biofilm and hyphae formation, proteinase, phospholipase, lipase and hemolytic activity, in 52 clinical isolates of C. tropicalis collected from five hospitals in four provinces of China. Some C. tropicalis tended to produce more hyphae than others in the same circumstance. Six C. tropicalis strains with different morphologies were injected into mice via the tail vein, and the survival proportions and fungal burdens of the strains were evaluated. Hyphal production by C tropicalis was associated with stronger virulence. RNA sequencing revealed that C tropicalis with more hyphae up-regulated several genes involved in morphological differentiation and oxidative response, including IF2, Abc1, and Sod2. It appears that hyphal formation plays a vital role in the pathogenicity of C. tropicalis, and interacts with the oxidative stress response to strengthen the organism's virulence. (C) 2016 Elsevier GmbH. All rights reserved.

引用

页码：65 / 72

页数：8

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