Development of polymerase chain reaction and comparison with in situ hybridization for the detection of Haemophilus parasuis in formalin-fixed, paraffin-embedded tissues

被引：7
作者：
Jung, K
Ha, Y
Kim, SH
Chae, C
机构：
[1] Seoul Natl Univ, Coll Vet Med, Dept Vet Pathol, Seoul 151742, South Korea
[2] Seoul Natl Univ, Sch Agr Biotechnol, Seoul 151742, South Korea
[3] Kyung Hee Univ, Grad Sch E W Med Sci, Dept Oncol, Kyounggi 449701, South Korea
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2004年 / 66卷 / 07期
关键词：
DNA extraction; Haemophilus parasuis; in situ hybridization; PCR;
D O I：
10.1292/jvms.66.841
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
DNA extraction and nested polymerase chain reaction (PCR) were developed for the detection of Haemophilus parasuis from formalin-fixed, paraffin-embedded tissues. The results for nested PCR were compared with those determined by in situ hybridization. The optimal results obtained show that use of xylene deparaffinization, digestion with proteinase K followed by nested PCR is a reliable detection method. A distinct positive signal was detected in 20 pigs naturally infected with H. parasuis by in situ hybrdization. The rate of agreement between nested PCR and in situ hybridization for the detection of H. parasuis in formalin-fixed, paraffin-embedded tissues was 100%. The nested PCR could be applied successfully to formalin-fixed, paraffin-embedded tissues for the detection of H. parasuis with bacterial isolation.
引用
收藏
页码：841 / 845
页数：5
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