Modified double-disc test for detection of extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae

被引：7

作者：

Kader, A. A. ^{[1
]}

Angamuthu, K. K. ^{[1
]}

Kamath, K. A. ^{[1
]}

Zaman, M. N. ^{[1
]}

机构：

[1] Almana Gen Hosp, Dept Clin Microbiol, Al Khobar 31952, Saudi Arabia

来源：

BRITISH JOURNAL OF BIOMEDICAL SCIENCE | 2006年 / 63卷 / 02期

关键词：

Escherichia coli; beta-lactamases; extended spectrum; Klebsiella pneumoniae;

D O I：

10.1080/09674845.2006.11732719

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

This study evaluates the performance of a modified double-disc test (MDDT) for the detection of extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Escherichia coli and Klebsiella pneumoniae. Ninety-six isolates of E. coli and 40 K. pneumoniae are studied for ESBL production by the National Committee for Clinical Laboratory Standards (NCCLS) combination disc tests and MDDT A total of 112 (82%) isolates (80 [83%] E. coli, 32 [80%] K. pneumoniae) were positive for ESBL by MDDT compared to 102 (75%; 72 [75%] E. coli and 30 [75%] K. pneumoniae) by the NCCLS method. In 10 (7.4%) isolates, ESBLs were detected only by MDDT Twenty-four (17.6%) isolates were negative for ESBL by both methods. The protocol described in this study provides a more sensitive approach than does the NCCLS method for ESBL detection in E. coli and K. pneumoniae.

引用

页码：51 / 54

页数：4

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