A lectin, PPL, was purified from the mushroom Pleurocybella porrigens. The results of SDS-PAGE, gel filtration, and MALDI-TOF-mass of PPL indicated that its molecular mass was 56 kDa, and it was composed of four 14 kDa subunits with no disulfide bonds. In hemagglutination inhibition assay, PPL exhibited the strongest binding specificity toward GalNAc among the mono- and oligo-saccharides tested. Among the glycoproteins, asialo-bovine submaxillary mucin (asialo-BSM) showed the strongest inhibitory effect. In surface plasmon resonance analysis, asialo-BSM, porcine stomach mucin (PSM), and BSM exhibited potent binding affinity. The complete amino acid sequence was determined by amino acid sequencing of intact and of enzyme-digested PPL. The cDNA of PPL was cloned from RNA extracted from the mushroom. The open reading frame of the cDNA of the protein consisted of 411 bp, encoding 137 amino acids. This is the first report of isolation of a lectin of the genus Pleurocybella.
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China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
Zhang, G. Q.
Sun, J.
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China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
Sun, J.
Wang, H. X.
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China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
Wang, H. X.
Ng, T. B.
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Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
机构:
China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
Wu, Yingying
Wang, Hexiang
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China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
Wang, Hexiang
Ng, Tzibun
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Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Hong Kong, Hong Kong, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
机构:Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China
Liu, QH
Wang, HX
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机构:Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China
Wang, HX
Ng, TB
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Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China
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China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Zhang, Weiwei
Tian, Guoting
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Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming 650223, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Tian, Guoting
Geng, Xueran
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China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Geng, Xueran
Zhao, Yongchang
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Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming 650223, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Zhao, Yongchang
Tzi Bun Ng
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Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Tzi Bun Ng
Zhao, Liyan
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Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
Zhao, Liyan
Wang, Hexiang
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China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R ChinaChina Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China