Purification, Characterization, and cDNA Cloning of a Lectin from the Mushroom Pleurocybella porrigens

被引：32
|
作者：
Suzuki, Tomohiro [1 ]
Amano, Yuko [2 ]
Fujita, Motohiro [2 ]
Kobayashi, Yuka [2 ]
Dohra, Hideo [3 ]
Hirai, Hirofumi [2 ]
Murata, Takeomi [2 ]
Usui, Taichi [2 ]
Morita, Tatsuya [2 ]
Kawagishi, Hirokazu [1 ,2 ]
机构：
[1] Shizuoka Univ, Grad Sch Sci & Technol, Dept Biosci, Suruga Ku, Shizuoka 4228529, Japan
[2] Shizuoka Univ, Fac Agr, Dept Appl Biol Chem, Suruga Ku, Shizuoka 4228529, Japan
[3] Shizuoka Univ, Inst Genet Res & Biotechnol, Suruga Ku, Shizuoka 4228529, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 03期
关键词：
mushroom; Pleurocybella porrigens; lectin; purification; cloning; HIGH-AFFINITY LECTIN; RICIN B-CHAIN; FRUITING BODIES; POLYPORUS-SQUAMOSUS; N-ACETYLLACTOSAMINE; CHROMATOGRAPHY; EXPRESSION;
D O I：
10.1271/bbb.80774
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A lectin, PPL, was purified from the mushroom Pleurocybella porrigens. The results of SDS-PAGE, gel filtration, and MALDI-TOF-mass of PPL indicated that its molecular mass was 56 kDa, and it was composed of four 14 kDa subunits with no disulfide bonds. In hemagglutination inhibition assay, PPL exhibited the strongest binding specificity toward GalNAc among the mono- and oligo-saccharides tested. Among the glycoproteins, asialo-bovine submaxillary mucin (asialo-BSM) showed the strongest inhibitory effect. In surface plasmon resonance analysis, asialo-BSM, porcine stomach mucin (PSM), and BSM exhibited potent binding affinity. The complete amino acid sequence was determined by amino acid sequencing of intact and of enzyme-digested PPL. The cDNA of PPL was cloned from RNA extracted from the mushroom. The open reading frame of the cDNA of the protein consisted of 411 bp, encoding 137 amino acids. This is the first report of isolation of a lectin of the genus Pleurocybella.
引用
收藏
页码：702 / 709
页数：8
相关论文
共 50 条
  • [1] An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens
    Suzuki, Tomohiro
    Nakamura, Luna
    Inayoshi, Satomi
    Tezuka, Yuki
    Ono, Akiko
    Choi, Jae-Hoon
    Dohra, Hideo
    Sasanami, Tomohiro
    Hirai, Hirofumi
    Kawagishi, Hirokazu
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2021, 85 (03) : 630 - 633
  • [2] Heterologous expression of a lectin from Pleurocybella porrigens (PPL) in Phanerochaete sordida YK-624
    Suzuki, Tomohiro
    Dohra, Hideo
    Omae, Saori
    Takeshima, Yoshino
    Choi, Jae-Hoon
    Hirai, Hirofumi
    Kawagishi, Hirokazu
    JOURNAL OF MICROBIOLOGICAL METHODS, 2014, 100 : 70 - 76
  • [3] Purification and Characterization of a Novel Lectin with Antiphytovirus Activities from the wild Mushroom Paxillus involutus
    Wang, Shou Xian
    Zhang, Guo Qing
    Zhao, Shuang
    Xu, Feng
    Zhou, Ying
    Geng, Xiao Li
    Liu, Yu
    Wang, He Xiang
    PROTEIN AND PEPTIDE LETTERS, 2013, 20 (07): : 767 - 774
  • [4] Cytotoxic fatty acid from Pleurocybella porrigens
    Hasegawa, Tatsllya
    Ishibashi, Mayumi
    Takata, Takanobu
    Takano, Fumihide
    Ohta, Tomihisa
    CHEMICAL & PHARMACEUTICAL BULLETIN, 2007, 55 (12) : 1748 - 1749
  • [5] Purification, characterization and cDNA cloning of a lectin from the brittle star Ophioplocus japonicus
    Imamichi, Yoshitaka
    Hikosaka, Kensuke
    Kawai, Naoki
    Koubaku, Naruchika
    Hosoi, Masatomi
    Mizuta, Shoshi
    Yokoyama, Yoshihiro
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2022, 262
  • [6] Purification and characterization of a novel lectin from the toxic wild mushroom Inocybe umbrinella
    Zhao, J. K.
    Wang, H. X.
    Ng, T. B.
    TOXICON, 2009, 53 (03) : 360 - 366
  • [7] Purification and characterization of a lectin from the toxic mushroom Amanita pantherina
    Zhuang, C
    Murata, T
    Usui, T
    Kawagishi, H
    Kobayashi, K
    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1996, 1291 (01): : 40 - 44
  • [8] Purification, Characterization, and cDNA Cloning of a Novel Lectin from the Green Alga, Codium barbatum
    Praseptiangga, Danar
    Hirayama, Makoto
    Hori, Kanji
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2012, 76 (04) : 805 - 811
  • [9] Chemical elucidation of acute encephalopathy by ingestion of angel-wing mushroom (Pleurocybella porrigens)-involvement of three constituents in onset
    Kawagishi, Hirokazu
    PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 2023, 99 (07): : 191 - 197
  • [10] Purification and characterization of a lectin from the mushroom Mycoleptodonoides aitchisonii
    Kawagishi, H
    Takagi, J
    Taira, T
    Murata, T
    Usui, T
    PHYTOCHEMISTRY, 2001, 56 (01) : 53 - 58
12345