In vitro evaluation of anti-diabetic activity and cytotoxicity of chemically analysed Ocimum basilicum extracts

被引：53

作者：

Kadan, Sleman ^{[1
,2
]}

Saad, Bashar ^{[1
,3
]}

Sasson, Yoel ^{[2
]}

Zaid, Hilal ^{[1
,3
]}

机构：

[1] Al Qasemi Acad, Qasemi Res Ctr, POB 124, IL-30100 Baqa El Gharbia, Israel

[2] Hebrew Univ Jerusalem, Inst Chem, Casali Ctr Appl Chem, IL-91904 Jerusalem, Israel

[3] Arab Amer Univ Jenin, Fac Arts & Sci, Jenin, Palestine

来源：

FOOD CHEMISTRY | 2016年 / 196卷

关键词：

Ocimum basilicum; Chemical composition; GLUT4; GC/MS; INDUCED INSULIN-RESISTANCE; STIMULATES GLUCOSE-UPTAKE; ESSENTIAL OILS; ACID; ADIPOCYTES; INHIBITION; TRANSPORT;

D O I：

10.1016/j.foodchem.2015.10.044

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim of this study was to evaluate the role of glucose transporter-4 (GLUT4) in the anti-diabetic effects of methanol, hexane and dichloromethane extracts of the aerial parts of Ocimum basilicum (OB) and to analyze their phytochemical composition. Phytochemical analysis of the three extracts by GC/MS using the silylation derivatization technique revealed 53 compounds, 17 of them were found for the first time in OB. Cytotoxic and anti-diabetic properties of the extracts were evaluated using L6-GLUT4myc muscle cells stably expressing myc epitope at the exofacial loop (GLUT4). No cytotoxic effects were observed in treated cells up to 0.25 mg/ml extract as measured with MTT and LDH-leakage assays. GLUT4 translocation to the plasma membrane was elevated by 3.5 and 7 folds (-/+ insulin) after treatment with OB extracts for 20 h. Our findings suggest that the observed anti-diabetic properties of OB extracts are possibly mediated in part through one or more of the 17 new identified compound. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：1066 / 1074

页数：9

共 45 条

[1] Chemical Composition, Antioxidant, Anti-Inflammatory and Anti-Proliferative Activities of Essential Oils of Plants from Burkina Faso [J].

Bayala, Bagora ;

Bassole, Imael Henri Nestor ;

Gnoula, Charlemagne ;

Nebie, Roger ;

Yonli, Albert ;

Morel, Laurent ;

Figueredo, Gilles ;

Nikiema, Jean-Baptiste ;

Lobaccaro, Jean-Marc A. ;

Simpore, Jacques .

PLOS ONE, 2014, 9 (03)

[2] Caffeic acid, naringenin and quercetin enhance glucose-stimulated insulin secretion and glucose sensitivity in INS-1E cells [J].

Bhattacharya, S. ;

Oksbjerg, N. ;

Young, J. F. ;

Jeppesen, P. B. .

DIABETES OBESITY & METABOLISM, 2014, 16 (07) :602-612

[3] Role of Ocimum basilicum L. in prevention of ischemia and reperfusion-induced cerebral damage, and motor dysfunctions in mice brain [J].

Bora, Kundan Singh ;

Arora, Shruti ;

Shri, Richa .

JOURNAL OF ETHNOPHARMACOLOGY, 2011, 137 (03) :1360-1365

[4] Glucose transporter recycling in response to insulin is facilitated by myosin Myo1c [J].

Bose, A ;

Guilherme, A ;

Robida, SI ;

Nicoloro, SMC ;

Zhou, QL ;

Jiang, ZY ;

Pomerleau, DP ;

Czech, MP .

NATURE, 2002, 420 (6917) :821-824

[5]

Chatterjee A., 1982, J NEMATOLOGY, V14

[6]

Croze M., 2015, BRIT J NUTR, V20, P14

[7]

Dev N., 2011, J SCI RES, V3, P10

[8] Hypoglycemic effect of basil (Ocimum basilicum) aqueous extract is mediated through inhibition of α-glucosidase and α-amylase activities: An in vitro study [J].

El-Beshbishy, H. A. ;

Bahashwan, S. A. .

TOXICOLOGY AND INDUSTRIAL HEALTH, 2012, 28 (01) :42-50

[9] Human hippocampal energy metabolism is impaired during cognitive activity in a lipid infusion model of insulin resistance [J].

Emmanuel, Yaso ;

Cochlin, Lowri E. ;

Tyler, Damian J. ;

De Jager, Celeste A. ;

Smith, A. David ;

Clarke, Kieran .

BRAIN AND BEHAVIOR, 2013, 3 (02) :134-144

[10] Leukocyte antigen-related inhibition attenuates palmitate-induced insulin resistance in muscle cells [J].

Gorgani-Firuzjaee, Sattar ;

Bakhtiyari, Salar ;

Golestani, Abolfazl ;

Meshkani, Reza .

JOURNAL OF ENDOCRINOLOGY, 2012, 215 (01) :71-77

← 1 2 3 4 5 →