Anthozoa red fluorescent protein in biosensing

被引：19
作者：
Shrestha, Suresh [1 ]
Deo, Sapna K. [1 ]
机构：
[1] Indiana Univ Purdue Univ, Dept Chem & Chem Biol, Indianapolis, IN 46202 USA
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2006年 / 386卷 / 03期
关键词：
red flourescent protein; bioanalytical methods; biosensing; FRET; BRET;
D O I：
10.1007/s00216-006-0652-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The identification and cloning of a red fluorescent protein (DsRed) obtained from Anthozoa corals has provided an alternative to commonly used green fluorescent proteins (GFPs) in bioanalytical and biomedical research. DsRed in tandem with GFPs has enhanced the feasibility of multicolor labeling studies. Properties of DsRed, for example high photostability, red-shifted fluorescence emission, and stability to pH changes have proven valuable in its use as a fluorescent tag in cell-biology applications. DsRed has some limitations, however. Its slow folding and tendency to form tetramers have been a hurdle. Several different mutational studies have been performed on DsRed to overcome these problems. In this paper, applications of DsRed in biosensing, specifically in FRET/BRET assays, whole-cell assays, and in biosensors, is discussed. In the future, construction of DsRed mutants with unique characteristics will further expand its applications in bioanalysis.
引用
收藏
页码：515 / 524
页数：10
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