Characterization of Achromobacter Species in Cystic Fibrosis Patients: Comparison of blaOXA-114 PCR Amplification, Multilocus Sequence Typing, and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

被引：12

作者：

Rodrigues, Elenice R. A. ^{[1
]}

Ferreira, Alex G. ^{[1
]}

Leao, Robson S. ^{[1
]}

Leite, Cassiana C. F. ^{[1
]}

Carvalho-Assef, Ana Paula ^{[3
]}

Albano, Rodolpho M. ^{[2
]}

Marques, Elizabeth A. ^{[1
]}

机构：

[1] Univ Estado Rio de Janeiro, Fac Ciencias Med, Dept Microbiol Imunol & Parasitol, BR-20550011 Rio De Janeiro, Brazil

[2] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Dept Bioquim, BR-20550011 Rio De Janeiro, Brazil

[3] Inst Oswaldo Cruz, Lab Pesquisa Infeccao Hosp, BR-20001 Rio De Janeiro, Brazil

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 12期

关键词：

MALDI-TOF MS; IDENTIFICATION; XYLOSOXIDANS; MICROBIOLOGY; YEASTS;

D O I：

10.1128/JCM.02197-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Molecular methodologies were used to identify 28 Achromobacter spp. from patients with cystic fibrosis (CF). Multilocus sequence typing (MLST) identified 17 Achromobacter xylosoxidans isolates (all bla(OXA-114) positive), nine Achromobacter ruhlandii isolates (all bla(OXA-114) positive), one Achromobacter dolens isolate, and one Achromobacter insuavis isolate. All less common species were misidentified as A. xylosoxidans by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Chronic colonization by clonally related A. ruhlandii isolates was demonstrated.

引用

页码：3894 / 3896

页数：3

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