Cloning and expression of two plant proteins: similar antimicrobial activity of native and recombinant form

被引：14
作者：
Capparelli, R.
Palumbo, D.
Iannaccone, M.
Ventimiglia, I.
Di Salle, E.
Capuano, F.
Salvatore, P.
Amoroso, M. G.
机构：
[1] Univ Naples Federico II, Sch Biotechnol Sci, I-80055 Naples, Italy
[2] Univ Naples Federico II, Dipartimento Biochim & Biotecnol Med, I-80131 Naples, Italy
[3] Ist Zooprofilatt Sperimental Mezzogiorno, I-80055 Naples, Italy
[4] Univ Naples Federico II, Sch Biotechnol Sci, Dept Cellular & Mol Pathol L Califano, I-80131 Naples, Italy
来源：
BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 13期
关键词：
antimicrobial activity; innate immunity; recombinant puroindolines; refolding; membrane damage;
D O I：
10.1007/s10529-006-9031-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Antimicrobial peptides and proteins are being studied with increasing interest because of their broad range antimicrobial activity. Among plant antimicrobial proteins, the wheat seed polypeptides, puroindoline a and puroindoline b, are particularly interesting because of their established antibacterial activity. In this paper we describe different strategies used to clone His tagged and GST tagged puroindolines obtaining 1.5 mg recombinant protein from 1 l culture. The antimicrobial activity of recombinant and native puroindolines was comparable.
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收藏
页码：943 / 949
页数：7
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