Research advances of DNA aptasensors for foodborne pathogen detection

被引:77
作者
Wu, Wei [1 ,2 ]
Yu, Chundi [1 ]
Wang, Qi [1 ]
Zhao, Fangyuan [1 ]
He, Hong [3 ]
Liu, Chunzhao [2 ,4 ]
Yang, Qingli [1 ]
机构
[1] Qingdao Agr Univ, Coll Food Sci & Engn, Qingdao, Shandong, Peoples R China
[2] Qingdao Univ, Sch Mat Sci & Engn, Inst Biochem Engn, State Key Lab Biofibers & Ecotext, Qingdao, Shandong, Peoples R China
[3] Qingdao Univ, Affiliated Hosp, Clin Lab, Qingdao, Shandong, Peoples R China
[4] Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金; 美国国家科学基金会;
关键词
Aptamer; food safety; rapid detection; SELEX; Escherichia coli; Salmonella; WHOLE-CELL SELEX; ENTERICA SEROVAR TYPHIMURIUM; COLORIMETRIC DETECTION; RAPID DETECTION; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; IMPEDIMETRIC APTASENSOR; SILVER NANOPARTICLES; GRAPHENE OXIDE; SANDWICH ASSAY;
D O I
10.1080/10408398.2019.1636763
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Aptamers, referring to single-stranded DNA or RNA molecules can specifically recognize and bind to their targets. Based on their excellent specificity, sensitivity, high affinity, and simplicity of modification, aptamers offer great potential for pathogen detection and biomolecular screening. This article reviews aptamer screening technologies and aptamer application technologies, including gold-nanoparticle lateral flow assays, fluorescence assays, electrochemical assays, colorimetric assays, and surface-enhanced Raman assays, in the detection of foodborne pathogens. Although notable progress (more rapid, sensitive, and accurate) has been achieved in the field, challenges and drawbacks in their applications still remain to be overcome.
引用
收藏
页码:2353 / 2368
页数:16
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