Emodin-mediated cross-linking enhancement for extracellular matrix homeostasis

被引：3

作者：

Jian, Lihua ^{[1
]}

Zhang, Chen ^{[1
]}

Chen, Guangfeng ^{[1
]}

Shi, Xiujuan ^{[1
]}

Qiu, Yu ^{[1
]}

Xue, Yunyun ^{[1
]}

Yang, Shuzhang ^{[1
]}

Lu, Lixia ^{[1
]}

Yuan, Qionglan ^{[1
]}

Xu, Guotong ^{[1
]}

Ying, Ming ^{[1
,2
]}

Liu, Xiaoqing ^{[1
,2
]}

机构：

[1] Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Shanghai 200092, Peoples R China

[2] Shenzhen Univ, Coll Life Sci, Shenzhen, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 446卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Emodin; Extracellular matrix (ECM); Lysyl oxidase-like 1 (LOXL1); Cross-linking; LYSYL OXIDASE; BIOLOGICAL ROLES; SKIN EQUIVALENT; IN-VITRO; COLLAGEN; PROTEIN; GENE; MICE; FIBULIN-5; FIBERS;

D O I：

10.1016/j.bbrc.2014.03.052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extracellular matrix (ECM) is an essential element of mammalian organisms, and its cross-linking formation plays a vital role in ECM development and postnatal homeostasis. Defects in cross-link formation caused by aging, genetic, or environmental factors are known to cause numerous diseases in mammals. To augment the cross-linking formation of ECM, the present study established a ZsGreen reporter system controlled by the promoter of lysyl oxidase-like I gene (L0XL1), which serves as both a scaffold element and a cross-linking enzyme in the ECM. By using this system in a drug screen, we identified erhodin as a strong enhancer of LOXL1 expression that promoted cross-linking formation of ECM in all the tested systems, including human fibroblast cells, cultured human skin tissues, and animals that received long-term emodin treatment. Collectively, the results suggest that emodin may serve as an effective drug or supplement for ECM homeostasis. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：1022 / 1028

页数：7

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