Copper Ions Interfere with the Reduction of the Water-Soluble Tetrazolium Salt-8

被引：24

作者：

Semisch, Annetta ^{[1
]}

Hartwig, Andrea ^{[1
]}

机构：

[1] Karlsruhe Inst Technol, Inst Appl Biosci, Dept Food Chem & Toxicol, D-76131 Karlsruhe, Germany

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 2014年 / 27卷 / 02期

关键词：

VIABILITY; TOXICITY; NADH;

D O I：

10.1021/tx400414c

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Metabolic activity as a measure of cell viability is frequently determined using the water-soluble tetrazolium salt 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt (WST-8), commercially available as CCK-8 reagent. In this study, CCK-8 was investigated with respect to its suitability for investigating nano- and microscale copper oxide (CuO NP and CuO MP) as well as water-soluble copper chloride (CuCl2). The results were compared to cell number and colony forming ability. Our data demonstrate that the CCK-8 assay overestimates the loss of metabolic activity by CuCl2 and CuO NP, because of interference by copper ions with the reduction of the dye.

引用

页码：169 / 171

页数：3

共 11 条

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