Stabilization of microtubules by cevipabulin

被引：9
作者：
Nasrin, Syeda Rubaiya [1 ]
Kabir, Arif Md. Rashedul [2 ]
Konagaya, Akihiko [3 ]
Ishihara, Tsukasa [4 ]
Sada, Kazuki [1 ,2 ]
Kakugo, Akira [1 ,2 ]
机构：
[1] Hokkaido Univ, Grad Sch Chem Sci & Engn, Sapporo, Hokkaido 0600810, Japan
[2] Hokkaido Univ, Fac Sci, Sapporo, Hokkaido 0600810, Japan
[3] Tokyo Inst Technol, Dept Computat Intelligence & Syst Sci, Yokohama, Kanagawa 2268502, Japan
[4] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, Ibaraki 3058566, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2019年 / 516卷 / 03期
基金：
日本学术振兴会;
关键词：
Microtubule; Cevipabulin; Persistence length; Kinesin; Motility assay; TUBULIN; MOLECULES; COMPOUND; TTI-237; AGENTS;
D O I：
10.1016/j.bbrc.2019.06.095
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the utility of cevipabulin as a stabilizing agent for microtubules. Cevipabulin-stabilized microtubules were more flexible compared to the microtubules stabilized by paclitaxel, the most commonly used microtubule stabilizing agent. Similar to the paclitaxel-stabilized microtubules, cevipabulin-stabilized microtubules were driven by kinesins in an in vitro gliding assay. The velocity of cevipabulin-stabilized microtubules was significantly higher than that of paclitaxel-stabilized microtubules. These findings will enrich the variety of microtubules with difference in mechanical and dynamic properties and widen their applications in nanotechnology. (C) 2019 Elsevier Inc. All rights reserved.
引用
收藏
页码：760 / 764
页数：5
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