Phenols removal by immobilized horseradish peroxidase

被引:111
作者
Alemzadeh, I. [1 ]
Nejati, S. [1 ]
机构
[1] Sharif Univ Technol, Dept Chem & Petr Engn, Tehran, Iran
关键词
Horseradish peroxidase (HRP); Hydrogen peroxide; Calcium alginate; Encapsulation; Phenol; WASTE-WATER; SOYBEAN PEROXIDASE; AQUEOUS PHENOL; MODEL;
D O I
10.1016/j.jhazmat.2008.12.026
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Application of immobilized horseradish peroxidase (HRP) in porous calcium alginate (ca-alginate) for the purpose of phenol removal is reported. The optimal conditions for immobilization of HRP in ca-alginate were identified. Gelation (encapsulation) was optimized at 1.0% (w/v) sodium alginate in the presence of 5.5% (w/v) of calcium chloride. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Increasing initial phenol concentration results in a decrease in % conversion. The highest conversion belongs to phenol concentration of 2 mM. Investigation into time course of phenol removal for both encapsulated and free enzymes showed that encapsulated enzyme had lower efficiency in comparison with the same concentration of free enzyme; however the capsules were reusable up to four cycles without any changes in their retention activity. Increasing enzyme concentration from 0.15 to 0.8 units/g alginate results in gradual increase in phenol removal. The ratio of hydrogen peroxide/phenol at which highest phenol removal obtained is found to be dependent on initial phenol concentration and in the solution of 2 and 8 mM phenol it was 1.15 and 0.94 respectively. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:1082 / 1086
页数:5
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