Comparison of fractionation techniques to obtain prebiotic galactooligosaccharides

被引：107

作者：

Hernandez, Oswaldo ^{[2
]}

Ruiz-Matute, Ana I. ^{[2
]}

Olano, Agustin ^{[1
]}

Javier Moreno, F. ^{[1
]}

Luz Sanz, M. ^{[2
]}

机构：

[1] CSIC, Inst Fermentac Ind, Madrid 28006, Spain

[2] CSIC, Inst Quim Organ Gen, E-28006 Madrid, Spain

来源：

INTERNATIONAL DAIRY JOURNAL | 2009年 / 19卷 / 09期

关键词：

GALACTO-OLIGOSACCHARIDES; POPULATION; ADSORPTION; TREHALOSE; LACTOSE;

D O I：

10.1016/j.idairyj.2009.03.002

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Four different fractionation techniques for obtaining galactooligosaccharides (GOS) free from digestible carbohydrates were compared. Diafiltration did not show any selectivity among mono-, di- and oligosaccharides, whereas yeast treatment to remove monosaccharides allowed a high recovery of GOS and disaccharides. Activated charcoal treatment showed a different selectivity in GOS recovery depending on the concentration of ethanolic water solutions used (1-15%). Ethanolic solutions of 8% led to a high recovery of GOS (similar to 90%), but 20% of disaccharides were also recovered, whilst solutions with 10% ethanol allowed almost complete removal of disaccharides, but only similar to 53% of GOS trisaccharides were recovered. Finally, the purest GOS fractions (degree of polymerization up to 8) were obtained using size exclusion chromatography. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：531 / 536

页数：6

共 27 条

[1]

Akpinar O, 2008, J FOOD AGRIC ENVIRON, V6, P55

[2] Effects of prebiotic oligosaccharides and trehalose on growth and production of bacteriocins by lactic acid bacteria [J].