MICROFLUIDIC IMPEDANCE FLOW CYTOMETRY FOR SINGLE-CELL PHYSIOLOGY STATES ANALYSIS OF TUMOR CELLS TREATED WITH CHEMOTHERAPY DRUGS

被引：2

作者：

Yuan, Xueyuan ^{[1
]}

Li, Tianyi ^{[1
]}

Yu, Flora ^{[2
]}

Han, Chengwu ^{[3
]}

Xing, Xiaoxing ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, Beijing, Peoples R China

[2] Univ Texas Dallas, Sch Nat Sci & Math, Dallas, TX USA

[3] China Japan Friendship Hosp, Beijing, Peoples R China

来源：

2022 IEEE 35TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS CONFERENCE (MEMS) | 2022年

基金：

中国国家自然科学基金;

关键词：

Impedance flow cytometry; cellular states; impedance feature; chemotherapeutic drug;

D O I：

10.1109/MEMS51670.2022.9699586

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

This paper reveals comprehensive cellular states post chemotherapeutic drug treatment measured by impedance flow cytometry beyond viability change. We map the impedance features to cell cycle arrests at G1/S and G2/M phase and apoptosis for lung cancer cells treated by paclitaxel (TAX) and gemcitabine (GEM). We find that the normalized 500kHz amplitude of cells treated with paclitaxel is higher than the control group, and these cells have significant negative phase shifts. When the cells are treated with gemcitabine, the amplitude change and phase shift are generally less than that of TAX-treated cells. With the drug concentration and exposure time increasing (24h to 48h), cells begin to undergo apoptosis, and the 500kHz amplitude of cells constantly decrease. In the G1/S and G2/M phase, the opacity of cells showed opposite changes, increasing for TAX-treated cells while decreasing for GEM-treated cells.

引用

页码：880 / 883

页数：4

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