Activity of cholinesterases, pyruvate kinase and adenosine deaminase in rats experimentally infected by Fasciola hepatica: Influences of these enzymes on inflammatory response and pathological findings

被引：27

作者：

Baldissera, Matheus D. ^{[1
]}

Bottari, Nathieli B. ^{[2
]}

Mendes, Ricardo E. ^{[3
]}

Schwertz, Claiton I. ^{[3
]}

Lucca, Neuber J. ^{[3
]}

Dalenogare, Diessica ^{[2
]}

Bochi, Guilherme V. ^{[4
]}

Moresco, Rafael N. ^{[4
]}

Morsch, Vera M. ^{[2
]}

Schetinger, Maria R. C. ^{[2
]}

Rech, Virginia C. ^{[5
]}

Jaques, Jeandre A. ^{[7
]}

Da Silva, Aleksandro S. ^{[6
]}

机构：

[1] Univ Fed Santa Maria, Dept Microbiol & Parasitol, BR-97119900 Santa Maria, RS, Brazil

[2] Univ Fed Santa Maria, Dept Biochem & Mol Biol, BR-97119900 Santa Maria, RS, Brazil

[3] IFC, Lab Vet Pathol, Concordia, SC, Brazil

[4] Univ Fed Santa Maria, Dept Clin & Toxicol Anal, BR-97119900 Santa Maria, RS, Brazil

[5] Ctr Univ Franciscano, Lab Nanosci, Santa Maria, RS, Brazil

[6] UDESC, Dept Anim Sci, Chapeco, SC, Brazil

[7] Univ Fed Mato Grosso Sul UFMS, Ctr Ciencias Biol Saude, Lab Gen Biochem & Microorganisms, Campo Grande, MS, Brazil

来源：

PATHOLOGY RESEARCH AND PRACTICE | 2015年 / 211卷 / 11期

关键词：

Fosciola hepatica; BChE; AChE; ADA; PK; Hepatic function; ACETYLCHOLINESTERASE ACTIVITY; ALLOSTERIC REGULATION; INTERFERON-GAMMA; IMMUNE-RESPONSE; LIVER; ENERGY; BRAIN; SERUM; BUTYRYLCHOLINESTERASE; INTERLEUKIN-4;

D O I：

10.1016/j.prp.2015.09.006

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The aim of this study was to investigate acetylcholinesterase (AChE) in total blood and liver tissue; butyrylcholinesterase (BChE) in serum and liver tissue; adenosine deaminase (ADA) in serum and liver tissue; and pyruvate kinase (P1<) in liver tissue of rats experimentally infected by Fasciola hepatica. Animals were divided into two groups with 12 animals each, as follows: group A (uninfected) and group B (infected). Samples were collected at 20 (A(1) and B-1; n=6 each) and 150 (A(2) and B-2; n=6 each) days post-infection (P1). Infected animals showed an increase in AChE activity in whole blood and a decrease in AChE activity in liver homogenates (P< 0.05) at 20 and 150 days P1. BChE and P1< activities were decreased (P< 0.05) in serum and liver homogenates of infected animals at 150 days P1. ADA activity was decreased in serum at 20 and 150 days P1, wh)le in liver homogenates it was only decreased at 150 days P1 (P< 0.05). Aspartate aminotransferase and alanine aminotransferase activities in serum were increased (P< 0.05), while concentrations of total protein and albumin were decreased (P< 0.05) when compared to control. The histological analysis revealed fibrous perihepatitis and necrosis. Therefore, we conclude that the liver fluke is associated with cholinergic and purinergic dysfunctions, which in turn may influence the pathogenesis of the disease. (C) 2015 Elsevier GmbH. All rights reserved.

引用

页码：871 / 876

页数：6

共 48 条

[11]

Da Silva AS, 2013, ACTA SCI VET, V41

[12] Neurobiology of butyrylcholinesterase [J].

Darvesh, S ;

Hopkins, DA ;

Geula, C .

NATURE REVIEWS NEUROSCIENCE, 2003, 4 (02) :131-138

[13] Butyrylcholinesterase activity in multiple sclerosis neuropathology [J].

Darvesh, Sultan ;

LeBlanc, Andrea M. ;

Macdonald, Ian R. ;

Reid, George A. ;

Bhan, Virender ;

Macaulay, Robert J. ;

Fisk, John D. .

CHEMICO-BIOLOGICAL INTERACTIONS, 2010, 187 (1-3) :425-431

[14]

Das UN, 2007, MED SCI MONITOR, V13, pRA214

[15] Liver protein synthesis in physiology and in disease states [J].

De Feo, P ;

Lucidi, P .

CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, 2002, 5 (01) :47-50

[16] Adenosine Deamination sustains dendritic cell activation in inflammation [J].

Desrosiers, Melanie D. ;

Cembrola, Katherine M. ;

Fakir, Michael J. ;

Stephens, Leslie A. ;

Jama, Fatimina M. ;

Shameli, Afshin ;

Mehal, Wajahat Z. ;

Santamaria, Pere ;

Shi, Yan .

JOURNAL OF IMMUNOLOGY, 2007, 179 (03) :1884-1892

[17] The effect of curcumin in the ectonucleotidases and acetylcholinesterase activities in synaptosomes from the cerebral cortex of cigarette smoke-exposed rats [J].

dos Santos Jaques, Jeandre Augusto ;

Peres Rezer, Joao Felipe ;

Goncalves, Jamile Fabbrin ;

Spanevello, Roselia Maria ;

Gutierres, Jessie Martins ;

Pimentel, Victor Camera ;

Thome, Gustavo Roberto ;

Morsch, Vera Maria ;

Chitolina Schetinger, Maria Rosa ;

Rosa Leal, Daniela Bitencourt .

CELL BIOCHEMISTRY AND FUNCTION, 2011, 29 (08) :703-707

[18] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].

ELLMAN, GL ;

COURTNEY, KD ;

ANDRES, V ;

FEATHERSTONE, RM .

BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&

[19] Cysteamine prevents inhibition of adenylate kinase caused by cystine in rat brain cortex [J].

Figueiredo, Vandre Casagrande ;

Feksa, Luciane Rosa ;

Duval Wannmacher, Clovis Milton .

METABOLIC BRAIN DISEASE, 2009, 24 (03) :373-381

[20]

Fredholm BB, 2011, ADV PHARMACOL, V61, P77, DOI 10.1016/B978-0-12-385526-8.00003-5

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