Common structural features of nucleic acid polymerases

被引:36
作者
Cramer, P [1 ]
机构
[1] Univ Munich, Inst Biochem, Gene Ctr, D-81377 Munich, Germany
关键词
D O I
10.1002/bies.10127
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Structures of multisubunit RNA polymerases strongly differ from the many known structures of single subunit DNA and RNA polymerases. However, in functional complexes of these diverse enzymes, nucleic acids take a similar course through the active center. This finding allows superposition of diverse polymerases and reveals features that are functionally equivalent. The entering DNA duplex is bent by almost 90degrees with respect to the exiting template-product duplex. At the point of bending, a dramatic twist between subsequent DNA template bases aligns the "coding" base with the binding site for the incoming nucleoside triphosphate (NTP). The NTP enters through an opening that is found in all polymerases, and, in most cases, binds between an alpha-helix and two catalytic metal ions. Subsequent phosphodiester bond formation adds a new base pair to the exiting template-product duplex, which is always bound from the minor groove side. All polymerases may undergo "induced fit" upon nucleic acid binding, but the underlying conformational changes differ. (C) 2002 Wiley Periodicals, Inc.
引用
收藏
页码:724 / 729
页数:6
相关论文
共 42 条
[1]   STRUCTURES OF DNA AND RNA-POLYMERASES AND THEIR INTERACTIONS WITH NUCLEIC-ACID SUBSTRATES [J].
ARNOLD, E ;
DING, JP ;
HUGHES, SH ;
HOSTOMSKY, Z .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1995, 5 (01) :27-38
[2]   DNA lesion bypass polymerases open up [J].
Beard, WA ;
Wilson, SH .
STRUCTURE, 2001, 9 (09) :759-764
[3]  
BONNER G, 1994, J BIOL CHEM, V269, P25129
[4]   Structural and functional insights provided by crystal structures of DNA polymerases and their substrate complexes [J].
Brautigam, CA ;
Steitz, TA .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1998, 8 (01) :54-63
[5]   Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus [J].
Bressanelli, S ;
Tomei, L ;
Roussel, A ;
Incitti, I ;
Vitale, RL ;
Mathieu, M ;
De Francesco, R ;
Rey, FA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (23) :13034-13039
[6]   Scanning mutagenesis reveals roles for helix N of the bacteriophage T7 RNA polymerase thumb subdomain in transcription complex stability, pausing, and termination [J].
Brieba, LG ;
Gopal, V ;
Sousa, R .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (13) :10306-10313
[7]   A mechanism for initiating RNA-dependent RNA polymerization [J].
Butcher, SJ ;
Grimes, JM ;
Makeyev, EV ;
Bamford, DH ;
Stuart, DL .
NATURE, 2001, 410 (6825) :235-240
[8]   Structure of a transcribing T7 RNA polymerase initiation complex [J].
Cheetham, GMT ;
Steitz, TA .
SCIENCE, 1999, 286 (5448) :2305-2309
[9]   Transcription regulation, initiation, and "DNA scrunching" by T7 RNA polymerase [J].
Cheetham, GMT ;
Jeruzalmi, D ;
Steitz, TA .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1998, 63 :263-267
[10]   Multisubunit RNA polymerases [J].
Cramer, P .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2002, 12 (01) :89-97