A Tandem Mass Spectrometric Method for Singlet Oxygen Measurement

被引：14

作者：

Karonen, Maarit ^{[1
]}

Mattila, Heta ^{[2
]}

Huang, Ping ^{[3
]}

Mamedov, Fikret ^{[3
]}

Styring, Stenbjoern ^{[3
]}

Tyystjarvi, Esa ^{[2
]}

机构：

[1] Univ Turku, Dept Chem, Lab Organ Chem & Chem Biol, Turku, Finland

[2] Univ Turku, Dept Biochem, SF-20500 Turku, Finland

[3] Uppsala Univ, Dept Chem, Angstrom Lab, Uppsala, Sweden

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 2014年 / 90卷 / 05期

基金：

芬兰科学院;

关键词：

ELECTROSPRAY-IONIZATION; PHOTOSYSTEM-II; PHOTOINHIBITION; MECHANISMS; PHOTOSYNTHESIS; OXIDATION; COMPLEX; TEMPO; YIELD; LIGHT;

D O I：

10.1111/php.12291

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Singlet oxygen, a harmful reactive oxygen species, can be quantified with the substance 2,2,6,6-tetramethylpiperidine (TEMP) that reacts with singlet oxygen, forming a stable nitroxyl radical (TEMPO). TEMPO has earlier been quantified with electron paramagnetic resonance (EPR) spectroscopy. In this study, we designed an ultra-high-performance liquid chromatographictandem mass spectrometric (UHPLC-ESI-MS/MS) quantification method for TEMPO and showed that the method based on multiple reaction monitoring (MRM) can be used for the measurements of singlet oxygen from both nonbiological and biological samples. Results obtained with both UHPLC-ESI-MS/MS and EPR methods suggest that plant thylakoid membranes produce 3.7x10(-7) molecules of singlet oxygen per chlorophyll molecule in a second when illuminated with the photosynthetic photon flux density of 2000molm(-2)s(-1).

引用

页码：965 / 971

页数：7

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