Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors

被引：19
|
作者：
Kolesar, Jill [1 ,2 ]
Huang, Wei [1 ,3 ]
Eickhoff, Jens [1 ,4 ]
Hahn, Kristine [1 ,5 ]
Alberti, Dona [1 ,5 ]
Attia, Steven [1 ,5 ]
Schelman, William [1 ,5 ]
Holen, Kyle [1 ,5 ]
Traynor, Anne [1 ,5 ]
Ivy, Percy [6 ]
Wilding, George [1 ,5 ]
机构：
[1] Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53792 USA
[2] Univ Wisconsin, Sch Pharm, Madison, WI 53792 USA
[3] Univ Wisconsin, Sch Med & Publ Hlth, Dept Pathol, Madison, WI 53792 USA
[4] Univ Wisconsin, Sch Med & Publ Hlth, Dept Biostat, Madison, WI 53792 USA
[5] Univ Wisconsin, Sch Med & Publ Hlth, Dept Med, Madison, WI 53792 USA
[6] NCI, Canc Therapy Evaluat Program, Bethesda, MD 20892 USA
来源：
CANCER CHEMOTHERAPY AND PHARMACOLOGY | 2009年 / 64卷 / 01期
关键词：
Triapine (R); 3-Aminopyridine-2-carboxaldehyde thiosemicarbazone; Ribonucleotide reductase; Automated quantitative immunohistochemistry (AQUA); Quantitative real time PCR; PHASE-II; CELL-PROLIFERATION; R2; COMPONENT; IN-VITRO; CANCER; OVEREXPRESSION; GEMCITABINE; TRANSFORMATION; RESISTANCE; INCREASES;
D O I：
10.1007/s00280-008-0845-0
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP. Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA). Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 +/- A 0.94 SD, vs 0.41 +/- A 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 +/- A 1.49 SD, vs 0.29 +/- A 0.20 SD, respectively; p = 0.02). Protein and gene expression were moderately associated (Spearman's rank correlation = 0.30; p = 0.12). RRM2 gene and protein expression varies by tumor type.
引用
收藏
页码：79 / 86
页数：8
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