Fucoidans from brown algae Laminaria longipes and Saccharina cichorioides: Structural characteristics, anticancer and radiosensitizing activity in vitro

被引：50
作者：
Usoltseva, Roza V. [1 ]
Shevchenko, Natalia M. [1 ]
Malyarenko, Olesya S. [1 ]
Anastyuk, Stanislav D. [1 ]
Kasprik, Anna E. [1 ]
Zvyagintsev, Nikolay V. [1 ]
Ermakova, Svetlana P. [1 ]
机构：
[1] Russian Acad Sci, Far Eastern Branch, GB Elyakov Pacific Inst Bioorgan Chem, 159,100 Let Vladivostoku Prosp, Vladivostok 690022, Russia
来源：
CARBOHYDRATE POLYMERS | 2019年 / 221卷
基金：
俄罗斯科学基金会; 俄罗斯基础研究基金会;
关键词：
Laminaria; Saccharina; Fucoidan; Anticancer activity; Radiosensitizing effect; ANTITUMOR-ACTIVITY; JAPONICA; POLYSACCHARIDES; SULFATE;
D O I：
10.1016/j.carbpol.2019.05.079
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The sulfated alpha-L-fucans ScF and LlF were obtained from brown algae of the Laminariaceae family (Saccharina cichorioides and Laminaria longipes). According to spectroscopy NMR, the LlF fucan predominantly contained the -> 3)-alpha-L-Fucp (2SO(3)-)-(1 -> 4)-alpha-L-Fucp-(1 -> 2)-alpha-L-Fucp-(4SO(3)-)-(1 -> repeating units, with small amounts of disaccharide 1,4-linked fragments and 3-sulfated fucose residues. Mass spectrometric analysis revealed the presence of the following fragments in the fucan structure: -alpha-L-Fucp-(2SO(3)-)-(1 -> 4)-alpha-L-Fucp-(2SO(3)-)-(1 -> 3)-alpha-L-Fucp-(4SO(3)-); alpha-L-Fucp-(2,4SO(3)-)-(1 -> 3)-alpha-L-Fucp-(1 -> 3)-alpha-L-Fucp-(4SO(3)-); alpha-L-Fucp-(2SO(3)-)-(1 -> 2)-alpha-L-Fucp; alpha-L-Fucp-(2SO(3)-)-(1 -> 2)-alpha-L-Fucp-(4SO(3)-); alpha-L-Fucp-(2SO(3)-)-(1 -> 3)-alpha-L-Fucp; alpha-L-Fucp-(2,4SO(3)-)-(1 -> 3)-alpha-L-Fucp; alpha-L-Fucp-(4SO(3)-)-(1 -> 4)-alpha-L-Fucp; and alpha-L-Fucp-(4SO(3)-)-(1 -> 4)-alpha-L-Fucp-(2SO(3)-). Both ScF and LlF fucoidans inhibited colony formation and growth of melanoma and colon cancer cells and sensitize-tested cancer cells to X-ray radiation to a comparable degree.
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页码：157 / 165
页数：9
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