Escherichia coli Nissle 1917 enhances bioavailability of serotonin in gut tissues through modulation of synthesis and clearance

被引：73
作者：
Nzakizwanayo, Jonathan [1 ]
Dedi, Cinzia [1 ]
Standen, Guy [1 ]
Macfarlane, Wendy M. [1 ]
Patel, Bhavik A. [1 ]
Jones, Brian V. [1 ,2 ]
机构：
[1] Univ Brighton, Sch Pharm & Biomol Sci, Brighton BN2 4GJ, E Sussex, England
[2] Queen Victoria Hosp NHS Fdn Trust, E Grinstead RH19 3DZ, England
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
基金：
英国工程与自然科学研究理事会; 英国医学研究理事会;
关键词：
INTESTINAL EPITHELIAL-CELLS; CHAIN FATTY-ACIDS; ULCERATIVE-COLITIS; MAINTAINING REMISSION; COLONIC MOTILITY; CLINICAL-TRIAL; BOWEL; BRAIN; BACTERIA; SYSTEM;
D O I：
10.1038/srep17324
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Accumulating evidence shows indigenous gut microbes can interact with the human host through modulation of serotonin (5-HT) signaling. Here we investigate the impact of the probiotic Escherichia coli Nissle 1917 (EcN) on 5-HT signalling in gut tissues. Ex-vivo mouse ileal tissue sections were treated with either EcN or the human gut commensal MG1655, and effects on levels of 5-HT, precursors, and metabolites, were evaluated using amperometry and high performance liquid chromatography with electrochemical detection (HPLC-EC). Exposure of tissue to EcN cells, but not MG1655 cells, was found to increase levels of extra-cellular 5-HT. These effects were not observed when tissues were treated with cell-free supernatant from bacterial cultures. In contrast, when supernatant recovered from untreated ileal tissue was pre-incubated with EcN, the derivative cell-free supernatant was able to elevate 5-HT overflow when used to treat fresh ileal tissue. Measurement of 5-HT precursors and metabolites indicated EcN also increases intracellular 5-HTP and reduces 5-HIAA. The former pointed to modulation of tryptophan hydroxylase-1 to enhance 5-HT synthesis, while the latter indicates an impact on clearance into enterocytes through SERT. Taken together, these findings show EcN is able to enhance 5-HT bioavailability in ileal tissues through interaction with compounds secreted from host tissues.
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页数：13
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