Enumeration of an Extremely High Particle-to-PFU Ratio for Varicella-Zoster Virus

被引：60

作者：

Carpenter, John E. ^{[1
]}

Henderson, Ernesto P. ^{[1
]}

Grose, Charles ^{[1
]}

机构：

[1] Univ Iowa, Cent Microscopy Res Facil, Iowa City, IA 52242 USA

来源：

JOURNAL OF VIROLOGY | 2009年 / 83卷 / 13期

关键词：

HUMAN-MELANOMA CELLS; GLYCOPROTEIN C; EGRESS; ALPHAHERPESVIRUSES; TEGUMENT; VZV;

D O I：

10.1128/JVI.00081-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Varicella-zoster virus (VZV) is renowned for its low titers. Yet investigations to explore the low infectivity are hampered by the fact that the VZV particle-to-PFU ratio has never been determined with precision. Herein, we accomplish that task by applying newer imaging technology. More than 300 images were taken of VZV-infected cells on 4 different samples at high magnification. We enumerated the total number of viral particles within 25 cm(2) of the infected monolayer at 415 million. Based on these numbers, the VZV particle:PFU ratio was approximately 40,000:1 for a cell-free inoculum.

引用

页码：6917 / 6921

页数：5

共 21 条

[1]

ANDERSON DR, 1986, STAT CONCEPTS APPL

[2] SEPARATION OF INFECTIOUS VARICELLA-ZOSTER VIRUS FROM HUMAN EMBRYONIC LUNG FIBROBLASTS [J].

BRUNELL, PA .

VIROLOGY, 1967, 31 (04) :732-&

[3] Egress of light particles among filopodia on the surface of varicella-zoster virus-infected cells [J].

Carpenter, John E. ;

Hutchinson, Jennifer A. ;

Jackson, Wallen ;

Grose, Charles .

JOURNAL OF VIROLOGY, 2008, 82 (06) :2821-2835

[4] GENERATION OF VARICELLA-ZOSTER VIRUS (VZV) AND VIRAL MUTANTS FROM COSMID DNAS - VZV THYMIDYLATE SYNTHETASE IS NOT ESSENTIAL FOR REPLICATION IN-VITRO [J].

COHEN, JI ;

SEIDEL, KE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (15) :7376-7380

[5]

Cohen JI, 2001, ARCH VIR S, V17, P91

[6]

Flint SJ., 2008, Principles of Virology

[7] Egress of alphaherpesviruses: Comparative ultrastructural study [J].

Granzow, H ;

Klupp, BG ;

Fuchs, W ;

Veits, J ;

Osterrieder, N ;

Mettenleiter, TC .

JOURNAL OF VIROLOGY, 2001, 75 (08) :3675-3684

[8] VARICELLA-ZOSTER VIRUS - ISOLATION AND PROPAGATION IN HUMAN MELANOMA CELLS AT 36 AND 32-DEGREES-C [J].

GROSE, C ;

BRUNELL, PA .

INFECTION AND IMMUNITY, 1978, 19 (01) :199-203

[9] CELL-FREE VARICELLA-ZOSTER VIRUS IN CULTURED HUMAN-MELANOMA CELLS [J].

GROSE, C ;

PERROTTA, DM ;

BRUNELL, PA ;

SMITH, GC .

JOURNAL OF GENERAL VIROLOGY, 1979, 43 (APR) :15-27

[10]

Harland J, 1998, METH MOLEC MED, V10, P1, DOI 10.1385/0-89603-347-3:1

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