Elevated Levels of G-Quadruplex Formation in Human Stomach and Liver Cancer Tissues

被引：169

作者：

Biffi, Giulia ^{[1
]}

Tannahill, David ^{[1
]}

Miller, Jodi ^{[1
]}

Howat, William J. ^{[1
]}

Balasubramanian, Shankar ^{[1
,2
]}

机构：

[1] Univ Cambridge, Canc Res UK Cambridge Inst, Cambridge, England

[2] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

PLOS ONE | 2014年 / 9卷 / 07期

关键词：

SMALL-MOLECULE; BREAKPOINT REGION; LIGAND RHPS4; IN-VITRO; DNA; INSTABILITY; MOTIFS; TRANSLOCATION; EXPRESSION; CONSISTENT;

D O I：

10.1371/journal.pone.0102711

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Four-stranded G-quadruplex DNA secondary structures have recently been visualized in the nuclei of human cultured cells. Here, we show that BG4, a G-quadruplex-specific antibody, can be used to stain DNA G-quadruplex structures in patient-derived tissues using immunohistochemistry. We observe a significantly elevated number of G-quadruplex-positive nuclei in human cancers of the liver and stomach as compared to background non-neoplastic tissue. Our results suggest that G-quadruplex formation can be detected and measured in patient-derived material and that elevated G-quadruplex formation may be a characteristic of some cancers.

引用

页数：9

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