Elevated Levels of G-Quadruplex Formation in Human Stomach and Liver Cancer Tissues

被引：178

作者：

Biffi, Giulia ^{[1
]}

Tannahill, David ^{[1
]}

Miller, Jodi ^{[1
]}

Howat, William J. ^{[1
]}

Balasubramanian, Shankar ^{[1
,2
]}

机构：

[1] Univ Cambridge, Canc Res UK Cambridge Inst, Cambridge, England

[2] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

PLOS ONE | 2014年 / 9卷 / 07期

关键词：

SMALL-MOLECULE; BREAKPOINT REGION; LIGAND RHPS4; IN-VITRO; DNA; INSTABILITY; MOTIFS; TRANSLOCATION; EXPRESSION; CONSISTENT;

D O I：

10.1371/journal.pone.0102711

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Four-stranded G-quadruplex DNA secondary structures have recently been visualized in the nuclei of human cultured cells. Here, we show that BG4, a G-quadruplex-specific antibody, can be used to stain DNA G-quadruplex structures in patient-derived tissues using immunohistochemistry. We observe a significantly elevated number of G-quadruplex-positive nuclei in human cancers of the liver and stomach as compared to background non-neoplastic tissue. Our results suggest that G-quadruplex formation can be detected and measured in patient-derived material and that elevated G-quadruplex formation may be a characteristic of some cancers.

引用

页数：9

共 34 条

[1] Bloom syndrome, genomic instability and cancer:: the SOS-like hypothesis [J].

Amor-Gueret, Mounira .

CANCER LETTERS, 2006, 236 (01) :1-12

[2] Targeting the c-Kit Promoter G-quadruplexes with 6-Substituted Indenoisoquinolines [J].

Bejugam, Mallesham ;

Gunaratnam, Mekala ;

Mueller, Sebastian ;

Sanders, Deborah A. ;

Sewitz, Sven ;

Fletcher, Jonathan A. ;

Neidle, Stephen ;

Balasubramanian, Shankar .

ACS MEDICINAL CHEMISTRY LETTERS, 2010, 1 (07) :306-310

[3]

Biffi G, 2013, NAT CHEM, V5, P182, DOI [10.1038/nchem.1548, 10.1038/NCHEM.1548]

[4] The G-quadruplex-interactive molecule BRACO-19 inhibits tumor growth, consistent with telomere targeting and interference with telomerase function [J].

Burger, AM ;

Dai, FP ;

Schultes, CM ;

Reszka, AP ;

Moore, MJ ;

Double, JA ;

Neidle, S .

CANCER RESEARCH, 2005, 65 (04) :1489-1496

[5] Defective telomere lagging strand synthesis in cells lacking WRN helicase activity [J].

Crabbe, L ;

Verdun, RE ;

Haggblom, CI ;

Karlseder, J .

SCIENCE, 2004, 306 (5703) :1951-1953

[6] G-quartets 40 years later:: From 5′-GMP to molecular biology and supramolecular chemistry [J].

Davis, JT .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (06) :668-698

[7] DNA secondary structures and epigenetic determinants of cancer genome evolution [J].

De, Subhajyoti ;

Michor, Franziska .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2011, 18 (08) :950-U115

[8] Conserved elements with potential to form polymorphic G-quadruplex structures in the first intron of human genes [J].

Eddy, Johanna ;

Maizels, Nancy .

NUCLEIC ACIDS RESEARCH, 2008, 36 (04) :1321-1333

[9] XPD helicase structures and activities: Insights into the cancer and aging phenotypes from XPD mutations [J].

Fan, Li ;

Fuss, Jill O. ;

Cheng, Quen J. ;

Arvai, Andrew S. ;

Hammel, Michal ;

Roberts, Victoria A. ;

Cooper, Priscilla K. ;

Tainer, John A. .

CELL, 2008, 133 (05) :789-800

[10] The G-quadruplex ligand telomestatin inhibits POT1 binding to Telomeric sequences in vitro and induces GFP-POT1 dissociation from telomeres in human cells [J].

Gomez, Dennis ;

O'Donohue, Marie-Francoise ;

Wenner, Thomas ;

Douarre, Cine ;

Macadre, Jerome ;

Koebel, Pascale ;

Giraud-Panis, Marie-Josephe ;

Kaplan, Herve ;

Kolkes, Alain ;

Shin-ya, Kazuo ;

Riou, Jean-Francois .

CANCER RESEARCH, 2006, 66 (14) :6908-6912

← 1 2 3 4 →