A red-emitting fluorescence turn-on probe for the discrimination of cysteine from biothiols and its bioimaging applications in living cells

被引：34

作者：

Jiao, Shan ^{[1
,2
]}

He, Xu ^{[4
]}

Xu, Longbin ^{[1
]}

Ma, Pinyi ^{[1
]}

Liu, Chunming ^{[3
]}

Huang, Yibing ^{[4
]}

Sun, Ying ^{[1
]}

Wang, Xinghua ^{[1
]}

Song, Daqian ^{[1
]}

机构：

[1] Jilin Univ, Coll Chem, Qianjin St 2699, Changchun 130012, Jilin, Peoples R China

[2] Jilin Univ, Endodont Dept, Stomatol Hosp, Qinghua Rd 1500, Changchun 130021, Jilin, Peoples R China

[3] Changchun Normal Univ, Cent Lab, 677 North Changji Rd, Changchun 130032, Jilin, Peoples R China

[4] Jilin Univ, Coll Life Sci, Qianjin St 2699, Changchun 130012, Jilin, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 290卷

关键词：

Fluorescent probe; Red-emitting; Cysteine; Endogenous; Living cells; HIGHLY SELECTIVE DETECTION; SENSOR; GLUTATHIONE; HOMOCYSTEINE; CONJUGATE; DESIGN; DOTS; CYS;

D O I：

10.1016/j.snb.2019.03.119

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, we synthesized a red-emitting fluorescence turn-on probe (HSA) for specific detection of cysteine (Cys) over other biothiols (glutathione (GSH) and homocysteine (Hcy)). The acrylate group was chosen as the recognition site for specific discrimination of Cys. Absorption and emission spectra display a high selectivity towards Cys over GSH/Hcy, organic small-molecule interfering substances and anions, with a high sensitivity towards Cys (0.12 mu M) in 1% ethanol-containing PBS buffer solution (25 mM, pH=7.4). Density functional theory (DFT) and time-dependent DFT (TDDFT) calculations were conducted to clarify the luminescence mechanism of the probe. Furthermore, the probe was successfully applied to the determination of free Cys in human serum and the fluorescence imaging of endogenous Cys in living cells.

引用

页码：47 / 52

页数：6

共 38 条

[1] A colorimetric and fluorescent probe for detecting intracellular GSH [J].

Chen, Chunyang ;

Liu, Wei ;

Xu, Cong ;

Liu, Weisheng .

BIOSENSORS & BIOELECTRONICS, 2015, 71 :68-74

[2] Synthesis and evaluation of a new fluorescein and rhodamine B-based chemosensor for highly sensitive and selective detection of cysteine over other amino acids and its application in living cell imaging [J].

Chen, Hailang ;

Zhou, Baojing ;

Ye, Renlong ;

Zhu, Jing ;

Bao, Xiaofeng .

SENSORS AND ACTUATORS B-CHEMICAL, 2017, 251 :481-489

[3] Design strategies for lab-on-a-molecule probes and orthogonal sensing [J].

Chen, Kun ;

Shu, Qinghai ;

Schmittel, Michael .

CHEMICAL SOCIETY REVIEWS, 2015, 44 (01) :136-160

[4] Fluorescent and colorimetric probes for detection of thiols [J].

Chen, Xiaoqiang ;

Zhou, Ying ;

Peng, Xiaojun ;

Yoon, Juyoung .

CHEMICAL SOCIETY REVIEWS, 2010, 39 (06) :2120-2135

[5] Nanosensor Composed of Nitrogen-Doped Carbon Dots and Gold Nanoparticles for Highly Selective Detection of Cysteine with Multiple Signals [J].

Deng, Jianhui ;

Lu, Qiujun ;

Hou, Yuxin ;

Liu, Meiling ;

Li, Haitao ;

Zhang, Youyu ;

Yao, Shouzhuo .

ANALYTICAL CHEMISTRY, 2015, 87 (04) :2195-2203

[6] L-cysteine capped ZnS:Mn quantum dots for room-temperature detection of dopamine with high sensitivity and selectivity [J].

Diaz-Diestra, Daysi ;

Thapa, Bibek ;

Beltran-Huarac, Juan ;

Weiner, Brad R. ;

Morell, Gerardo .

BIOSENSORS & BIOELECTRONICS, 2017, 87 :693-700

[7] Molybdenum nitride/nitrogen-doped multi-walled carbon nanotubes hybrid nanocomposites as novel electrochemical sensor for detection L-cysteine [J].

Geng, Di ;

Li, Mian ;

Bo, Xiangjie ;

Guo, Liping .

SENSORS AND ACTUATORS B-CHEMICAL, 2016, 237 :581-590

[8] Ratiometric fluorescent nanosensors for selective detecting cysteine with upconversion luminescence [J].

Guan, Yunlong ;

Qu, Songnan ;

Li, Bin ;

Zhang, Liming ;

Ma, Heping ;

Zhang, Ligong .

BIOSENSORS & BIOELECTRONICS, 2016, 77 :124-130

[9] Mitochondria-Targeted Near-Infrared Fluorescent Off-On Probe for Selective Detection of Cysteine in Living Cells and in Vivo [J].

Han, Chunmiao ;

Yang, Huiran ;

Chen, Min ;

Su, Qianqian ;

Feng, Wei ;

Li, Fuyou .

ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (50) :27968-27975

[10] Improved Aromatic Substitution Rearrangement-Based Ratiometric Fluorescent Cysteine-Specific Probe and Its Application of Real-Time Imaging under Oxidative Stress in Living Zebrafish [J].

He, Longwei ;

Yang, Xueling ;

Xu, Kaixin ;

Lin, Weiying .

ANALYTICAL CHEMISTRY, 2017, 89 (17) :9567-9573

← 1 2 3 4 →