Identification of a major blast resistance gene in the rice cultivar 'Tetep'

被引:36
作者
Barman, SR
Gowda, M
Venu, RC
Chattoo, BB [1 ]
机构
[1] Maharaja Sayajirao Univ Baroda, Fac Sci, Dept Microbiol, Vadodara 390002, India
[2] Maharaja Sayajirao Univ Baroda, Fac Sci, Ctr Biotechnol, Vadodara 390002, India
关键词
Oryza sativa; Magnaporthe grisea; blast resistance; molecular markers; near-isogenic lines;
D O I
10.1111/j.1439-0523.2004.00982.x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Analysis of near-isogenic lines (NILs) indicated the presence of a novel resistance gene in the indica rice cultivar 'Tetep' which was highly resistant to the rice blast fungus Magnaporthe grisea. 'Tetep' was crossed to the widely used susceptible cultivar 'CO39' to generate the mapping population. A Mendelian segregation ratio of 3 : 1 for resistant to susceptible F-2 plants further confirmed the presence of a major dominant locus, in 'Tetep', conferring resistance to the blast fungal isolate B157, corresponding to the international race IC9. Simple sequence length polymorphism (SSLP) was used for molecular genetic analysis. The analysis revealed that the SSLP marker RM 246 was linked to a novel blast resistance gene designated Pi-tp(t) in 'Tetep'.
引用
收藏
页码:300 / 302
页数:3
相关论文
共 22 条
[1]  
Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P229, DOI DOI 10.1007/BF02672073
[2]  
Bonman J. M., 1988, Oryza, V25, P103
[3]   A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta [J].
Bryan, GT ;
Wu, KS ;
Farrall, L ;
Jia, YL ;
Hershey, HP ;
McAdams, SA ;
Faulk, KN ;
Donaldson, GK ;
Tarchini, R ;
Valent, B .
PLANT CELL, 2000, 12 (11) :2033-2045
[4]   A draft sequence of the rice genome (Oryza sativa L. ssp japonica) [J].
Goff, SA ;
Ricke, D ;
Lan, TH ;
Presting, G ;
Wang, RL ;
Dunn, M ;
Glazebrook, J ;
Sessions, A ;
Oeller, P ;
Varma, H ;
Hadley, D ;
Hutchinson, D ;
Martin, C ;
Katagiri, F ;
Lange, BM ;
Moughamer, T ;
Xia, Y ;
Budworth, P ;
Zhong, JP ;
Miguel, T ;
Paszkowski, U ;
Zhang, SP ;
Colbert, M ;
Sun, WL ;
Chen, LL ;
Cooper, B ;
Park, S ;
Wood, TC ;
Mao, L ;
Quail, P ;
Wing, R ;
Dean, R ;
Yu, YS ;
Zharkikh, A ;
Shen, R ;
Sahasrabudhe, S ;
Thomas, A ;
Cannings, R ;
Gutin, A ;
Pruss, D ;
Reid, J ;
Tavtigian, S ;
Mitchell, J ;
Eldredge, G ;
Scholl, T ;
Miller, RM ;
Bhatnagar, S ;
Adey, N ;
Rubano, T ;
Tusneem, N .
SCIENCE, 2002, 296 (5565) :92-100
[5]   ALLELISM OF BLAST RESISTANCE GENES IN NEAR-ISOGENIC LINES OF RICE [J].
INUKAI, T ;
NELSON, RJ ;
ZEIGLER, RS ;
SARKARUNG, S ;
MACKILL, DJ ;
BONMAN, JM ;
TAKAMURE, I ;
KINOSHITA, T .
PHYTOPATHOLOGY, 1994, 84 (11) :1278-1283
[6]  
*IRRI, 1995, PAR IRRI CROSS
[7]   Origin, dispersal, cultivation and variation of rice [J].
Khush, GS .
PLANT MOLECULAR BIOLOGY, 1997, 35 (1-2) :25-34
[8]  
Kosambi D. D., 1944, ANN EUGENICS, V12, P172
[9]  
LANDER E S, 1987, Genomics, V1, P174, DOI 10.1016/0888-7543(87)90010-3
[10]  
LING KC, 1969, PHYTOPATHOLOGY, V59, P339