Genomic investigation of a sequence type 67 Clostridium difficile causing community-acquired fulminant colitis in Hong Kong

被引：7

作者：

Cao, Huiluo ^{[1
,2
]}

Wong, Sally Cheuk-Ying ^{[1
,2
,3
]}

Yam, Wing-Cheong ^{[1
,2
,3
]}

Liu, Melissa Chun-Jiao ^{[1
,2
]}

Chow, Kin-Hung ^{[1
,2
]}

Wu, Alan Ka-Lun ^{[4
]}

Ho, Pak-Leung ^{[1
,2
,3
]}

机构：

[1] Univ Hong Kong, Carol Yu Ctr Infect, Hong Kong, Peoples R China

[2] Univ Hong Kong, Dept Microbiol, Hong Kong, Peoples R China

[3] Queen Mary Hosp, Dept Microbiol, Hong Kong, Peoples R China

[4] Pamela Youde Nethersole Eastern Hosp, Dept Clin Pathol, Hong Kong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY | 2019年 / 309卷 / 05期

关键词：

Hypervirulent; Clostridioides difficile; Binary toxin; Pathogenicity locus; Trehalose repressor; RIBOTYPE; 002; INFECTION; MORTALITY; ADULTS;

D O I：

10.1016/j.ijmm.2019.05.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In 2017, we identified a Clostridium difficile strain HKCD4 that caused community-acquired fulminant colitis in a previously healthy child. Phylogenetically, it belonged to Glade 2, sequence type 67 and was resistant to fluoroquinolone and tetracycline. The strain was pathogenicity locus and binary toxin positive. It has a mutation in the trehalose repressor treR leading to the L172I substitution that was previously reported in the epidemic ribotype 027 lineage. HKCD4 has a tcdB sequence that shared very high identities with 3 highly virulent reference strains. It has a CpG depleted genome that is characteristic of hypervirulent C. difficile. The emergence of ST67 lineage with molecular feature of hypervirulence in the community is concerning and emphasizes the need for full characterization of strains causing severe disease in patients without classical risk factors.

引用

页码：270 / 273

页数：4

共 22 条

[1]

Ashkar Ali A., 2002, Current Molecular Medicine (Hilversum), V2, P545, DOI 10.2174/1566524023362159

[2] The importance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for correct identification of Clostridium difficile isolated from chromID C. difficile chromogenic agar [J].

Chen, Jonathan H. K. ;

Cheng, Vincent C. C. ;

Wong, Oi-Ying ;

Wong, Sally C. Y. ;

So, Simon Y. C. ;

Yam, Wing-Cheong ;

Yuen, Kwok-Yung .

JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, 2017, 50 (05) :723-726

[3] Containment of Clostridium difficile infection without reduction in antimicrobial use in Hong Kong [J].

Cheng, V. C. C. ;

Chau, P. H. ;

So, S. Y. C. ;

Chen, J. H. K. ;

Poon, R. W. S. ;

Wong, S. C. Y. ;

Hung, I. F. N. ;

Lee, W. M. ;

Tai, J. W. M. ;

Ho, P. L. ;

Yam, W. C. ;

Yuen, K. Y. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2015, 34 (07) :1381-1386

[4] Clostridium difficile isolates with increased sporulation: emergence of PCR ribotype 002 in Hong Kong [J].

Cheng, V. C. C. ;

Yam, W. C. ;

Lam, O. T. C. ;

Tsang, J. L. Y. ;

Tse, E. Y. F. ;

Siu, G. K. H. ;

Chan, J. F. W. ;

Tse, H. ;

To, K. K. W. ;

Tai, J. W. M. ;

Ho, P. L. ;

Yuen, K. Y. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2011, 30 (11) :1371-1381

[5] Whole-genome sequencing data-based modeling for the investigation of an outbreak of community-associated methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit in Hong Kong [J].

Cheng, Vincent C. C. ;

Wong, Shuk-Ching ;

Cao, Huiluo ;

Chen, Jonathan H. K. ;

So, Simon Y. C. ;

Wong, Sally C. Y. ;

Sridhar, Siddharth ;

Yuen, Kwok-Yung ;

Ho, Pak-Leung .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2019, 38 (03) :563-573

[6] Surveillance of antibiotic resistance among common Clostridium difficile ribotypes in Hong Kong [J].

Chow, Viola C. Y. ;

Kwong, Thomas N. Y. ;

So, Erica W. M. ;

Ho, Yolanda I. I. ;

Wong, Sunny H. ;

Lai, Raymond W. M. ;

Chan, Raphael C. Y. .

SCIENTIFIC REPORTS, 2017, 7

[7] Dietary trehalose enhances virulence of epidemic Clostridium difficile [J].

Collins, J. ;

Robinson, C. ;

Danhof, H. ;

Knetsch, C. W. ;

van Leeuwen, H. C. ;

Lawley, T. D. ;

Auchtung, J. M. ;

Britton, R. A. .

NATURE, 2018, 553 (7688) :291-+

[8] Ribotypes and New Virulent Strains Across Europe [J].

Couturier, Jeanne ;

Davies, Kerrie ;

Gateau, Cecile ;

Barbut, Frederic .

UPDATES ON CLOSTRIDIUM DIFFICILE IN EUROPE: ADVANCES IN MICROBIOLOGY, INFECTIOUS DISEASES AND PUBLIC HEALTH, VOL 8, 2018, 1050 :45-58

[9] Evolutionary History of the Clostridium difficile Pathogenicity Locus [J].

Dingle, Kate E. ;

Elliott, Briony ;

Robinson, Esther ;

Griffiths, David ;

Eyre, David W. ;

Stoesser, Nicole ;

Vaughan, Alison ;

Golubchik, Tanya ;

Fawley, Warren N. ;

Wilcox, Mark H. ;

Peto, Timothy E. ;

Walker, A. Sarah ;

Riley, Thomas V. ;

Crook, Derrick W. ;

Didelot, Xavier .

GENOME BIOLOGY AND EVOLUTION, 2014, 6 (01) :36-52

[10] Clinical Clostridium difficile: Clonality and Pathogenicity Locus Diversity [J].

Dingle, Kate E. ;

Griffiths, David ;

Didelot, Xavier ;

Evans, Jessica ;

Vaughan, Alison ;

Kachrimanidou, Melina ;

Stoesser, Nicole ;

Jolley, Keith A. ;

Golubchik, Tanya ;

Harding, Rosalind M. ;

Peto, Tim E. ;

Fawley, Warren ;

Walker, A. Sarah ;

Wilcox, Mark ;

Crook, Derrick W. .

PLOS ONE, 2011, 6 (05)

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