Requirement of CD9 on the egg plasma membrane for fertilization

被引：548

作者：

Miyado, K

Yamada, G

Yamada, S

Hasuwa, H

Nakamura, Y

Ryu, F

Suzuki, K

Kosai, K

Inoue, K

Ogura, A

Okabe, M

Mekada, E ^{[1
]}

机构：

[1] Kurume Univ, Inst Life Sci, Fukuoka 8390861, Japan

[2] Kurume Univ, Res Ctr Innovat Canc Therapy, Fukuoka 8300011, Japan

[3] Kumamoto Univ, Ctr Anim Resources & Dev, Kumamoto 8600811, Japan

[4] Program Promot Basic Res Act Innovat Biosci, Tokyo 1050001, Japan

[5] St Marys Hosp, Fukuoka 8300047, Japan

[6] Natl Inst Infect Dis, Shinjuku Ku, Tokyo 1628640, Japan

[7] Osaka Univ, Genome Informat Res Ctr, Osaka 5650861, Japan

来源：

SCIENCE | 2000年 / 287卷 / 5451期

关键词：

D O I：

10.1126/science.287.5451.321

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

CD9 is an integral membrane protein associated with integrins and other membrane proteins, Mice lacking CD9 were produced by homologous recombination. Both male and female CD9(-/-) mice were born healthy and grew normally, However, the litter size from CD9(-/-) females was less than 2% of that of the wild type. In vitro fertilization experiments indicated that the cause of this infertility was due to;the failure of sperm-egg fusion. When sperm were injected into oocytes with assisted microfertilization techniques, however, the fertilized eggs developed to term. These results indicate that CD9 has a crucial role in sperm-egg fusion.

引用

页码：321 / 324

页数：4

共 31 条

[1] MOUSE EGG INTEGRIN ALPHA-6-BETA-1 FUNCTIONS AS A SPERM RECEPTOR [J].