Regulation of NGFI-A (Egr-1) gene expression by the POU domain transcription factor Brn-3a

被引：6
作者：
Smith, MD
Ensor, EA
Stohl, L
Wagner, JA
Latchman, DS
机构：
[1] UCL, Dept Mol Pathol, Windeyer Inst Med Sci, London W1P 6DB, England
[2] Cornell Univ Med Coll, Dept Neurol & Neurosci, New York, NY USA
来源：
MOLECULAR BRAIN RESEARCH | 1999年 / 74卷 / 1-2期
基金：
英国医学研究理事会;
关键词：
Brn-3; POU domain; neurotrophin; NGFI-A; immediate early;
D O I：
10.1016/S0169-328X(99)00271-5
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
NGFI-A is an immediate early gene (IEG) that is transcriptionally induced by nerve growth factor (NGF) in PC12 cells and has been implicated in a number of cellular responses. Studies have shown that elements within the first 106 base pairs of the NGFI-A promoter contribute to its induction by NGF in PC12 cells. One element, within the serum response element (SRE) bridge region, bears strong homology to a motif previously identified in promoters regulated by the Brn-3a POU domain transcription factor. We report here that Brn-3a activates the NGFI-A promoter in neurons (both primary and cell lines). Analysis revealed that this response requires sequences between positions -49 and -106. Whilst DNA-protein interaction studies failed to identify a site bound directly by Brn-3a, the data presented here suggest that Brn-3a may cooperate in the regulation of NGFl-A gene expression in neurons, possibly during the developmental switch between neurotrophin dependency that occurs during neurogenesis. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：117 / 125
页数：9
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