In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

被引：13
|
作者：
Zhu, QZ [1 ]
Xu, JG [1 ]
Guo, XQ [1 ]
Zheng, XY [1 ]
Li, WY [1 ]
Zhao, YB [1 ]
机构：
[1] XIAMEN UNIV,DEPT CHEM,RES LAB SEDC ANALYT SCI MAT & LIFE CHEM,XIAMEN 361005,PEOPLES R CHINA
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 1997年 / 53卷 / 12期
关键词：
photochemical reaction; fluorimetry; BSA detection; 9,10-anthraquinone;
D O I：
10.1016/S1386-1425(97)00123-6
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorometric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ was investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 x 10(-7) M BSA. The detection limit was 1.2 x 10(-10) M BSA and the relative standard deviation was 2.24% for the 1.42 x 10(-8) M BSA (n = 7). (C) 1997 Elsevier Science B.V.
引用
收藏
页码：2195 / 2200
页数：6
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