TNF-α augmented Porphyromonas gingivalis invasion in human gingival epithelial cells through Rab5 and ICAM-1

被引:23
作者
Kato, Yoshiko [1 ,2 ]
Hagiwara, Makoto [1 ]
Ishihara, Yuichi [2 ]
Isoda, Ryutaro [1 ]
Sugiura, Shinsuke [2 ]
Komatsu, Toshinori [1 ]
Ishida, Naoyuki [1 ,2 ]
Noguchi, Toshihide [2 ]
Matsushita, Kenji [1 ]
机构
[1] Natl Ctr Geriatr & Gerontol, Dept Oral Dis Res, Obu, Aichi 7478511, Japan
[2] Aichi Gakuin Univ, Dept Periodontol, Nagoya, Aichi, Japan
来源
BMC MICROBIOLOGY | 2014年 / 14卷
关键词
Porphyromonas gingivalis; Endocytosis; TNF-alpha; Rab5; ICAM-1; Persistent infection; NECROSIS-FACTOR-ALPHA; INTERCELLULAR-ADHESION MOLECULE-1; VEIN ENDOTHELIAL-CELLS; AGGRESSIVE PERIODONTITIS; ALPHA-5-BETA-1; INTEGRIN; CONTAINING PHAGOSOMES; SIGNAL-TRANSDUCTION; CYTOKINE PROFILES; MAMMALIAN-CELLS; EARLY ENDOSOMES;
D O I
10.1186/s12866-014-0229-z
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Tumor necrosis factor alpha (TNF-alpha) plays a central role in the initiation and maintenance of immune responses to periodontopathic bacteria. However, excess TNF-alpha leads to dysregulated immune responses and progression of periodontitis. Porphyromonas gingivalis (P. gingivalis) invades gingival epithelial cells and then multiplies and survives for a long period. Additionally, increment of TNF-alpha in periodontal sites is associated with a high prevalence of gram-negative anaerobes such as P. gingivalis. However, it has not been determined whether TNF-alpha affects invasion of P. gingivalis in periodontal tissues. Results: We examined the effect of TNF-alpha on invasion of P. gingivalis in gingival epithelial cells and clarified the mechanism by which TNF-alpha augments invasion of P. gingivalis. Invasion of P. gingivalis into Ca9-22 cells was augmented by stimulation with TNF-alpha and it was inhibited by treatment with an antibody to TNF receptor-1. TNF-alpha increased production of ICAM-1, and P. gingivalis invasion was inhibited by an antibody to ICAM-1 in Ca9-22 cells. Silencing of Rab5 mRNA inhibited P. gingivalis invasion. Furthermore, the JNK inhibitor SP600125 inhibited invasion of P. gingivalis and also decreased the active form of Rab5 in Ca9-22 cells. Conclusion: TNF-alpha augments invasion of P. gingivalis in human gingival epithelial cells through increment of ICAM-1 and activation of Rab5. These phenomena may contribute to persistent infection of P. ginigvalis and prolongation of immune responses in periodontal tissues.
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页数:13
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