Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation

被引：30
|
作者：
Selvakumar, P [1 ]
Ashakumary, L [1 ]
Helen, A [1 ]
Pandey, A [1 ]
机构：
[1] CSIR, REG RES LAB, BIOTECHNOL UNIT, TRIVANDRUM 695019, KERALA, INDIA
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1996年 / 23卷 / 06期
关键词：
D O I：
10.1111/j.1472-765X.1996.tb01346.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glucoamylases produced by Aspergillus niger grown on wheat bran in solid cultures were purified. Four different forms, GA I, GA I', GA II and GA III, were found having apparent molecular weights of 112000, 104000, 74000 and 61000 Da respectively. The enzymes are glycoproteins with a carbohydrate content of 16%, and optimal activity at 60 degrees C and pH 4.4. Activity was strongly inhibited by Hg2+ while Mn2+ and Fe2+ were stimulatory. The K-m values for the degradation of starch and maltose were 3.5 and 7.8 mg ml(-1), respectively.
引用
收藏
页码：403 / 406
页数：4
相关论文
共 50 条
  • [41] Global transcriptional response of Aspergillus niger in the process of glucoamylase fermentation
    Sui Y.-F.
    Ouyang L.-M.
    Chu J.
    Cao W.-Q.
    liang L.-F.
    Zhuang Y.-P.
    Cheng S.
    Norrman H.
    Zhang S.-L.
    zhang G.-Y.
    Bioresources and Bioprocessing, 2017, 4 (01)
  • [42] Solid-state fermentation with Aspergillus niger for cellobiase production
    Tsao, GT
    Xia, LM
    Cao, NJ
    Gong, CS
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2000, 84-6 (1-9) : 743 - 749
  • [43] Biochemical and molecular characterization of glucoamylase produced by the Aspergillus niger strain HPD-2
    Perez-Rodriguez, Fernando
    Pliego-Arreaga, Raquel
    Silva-Martinez, Guillermo Antonio
    Cervantes-Montelongo, Juan Antonio
    BIOTECNIA, 2024, 26 (01): : 5 - 15
  • [44] Purification and properties of two forms of glucoamylase from Aspergillus niger
    Amirul, AA
    Khoo, SL
    Nazalan, MN
    Razip, MS
    Azizan, MN
    FOLIA MICROBIOLOGICA, 1996, 41 (02) : 165 - 174
  • [45] COMPARATIVE STUDY FOR OXYGENASES PRODUCED BY Aspergillus niger, ATCC 9642, IN SOLID-STATE AND SUBMERGED FERMENTATION
    Flores-Flores, T. C.
    Gutierrez-Rojas, M.
    Revah, S.
    Favela-Torres, E.
    REVISTA MEXICANA DE INGENIERIA QUIMICA, 2011, 10 (02): : 189 - 207
  • [46] A STUDY OF THE INHIBITION OF THE ENZYME TANASE PRODUCED BY ASPERGILLUS NIGER GH1 IN SOLID STATE FERMENTATION
    Rodriguez Duran, Luis Victor
    Rodriguez Duran, Nadia A.
    Rodriguez Duran, Nubia Rocio
    Cruz Hernandez, Mario A.
    Aguilar Gonzalez, Cristobal Noe
    CIENCIAUAT, 2009, 3 (03) : 65 - 67
  • [47] Purification and characterization of a thermostable glucoamylase produced by Aspergillus flavus HBF34
    Koc, Oeznur
    Metin, Kubilay
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 9 (23): : 3414 - 3424
  • [48] Production and characterization of a highly active cellobiase from Aspergillus niger grown in solid state fermentation
    Muhammad Ibrahim Rajoka
    Muhammad Waheed Akhtar
    Atif Hanif
    A. M. Khalid
    World Journal of Microbiology and Biotechnology, 2006, 22 : 991 - 998
  • [49] OPTIMIZATION AND CHARACTERIZATION OF EXO-POLYGALACTURONASE BY Aspergillus niger CULTURED VIA SOLID STATE FERMENTATION
    Pagarra, Halifah
    Rahman, Roshanida A.
    Azelee, Nur Izyan Wan
    Illias, Rosli Md
    JURNAL TEKNOLOGI-SCIENCES & ENGINEERING, 2019, 81 (01): : 59 - 67
  • [50] Production and characterization of a highly active cellobiase from Aspergillus niger grown in solid state fermentation
    Rajoka, Muhammad Ibrahim
    Akhtar, Muhammad Waheed
    Hanif, Atif
    Khalid, A. M.
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2006, 22 (09): : 991 - 998
12345