Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation

被引：30
|
作者：
Selvakumar, P [1 ]
Ashakumary, L [1 ]
Helen, A [1 ]
Pandey, A [1 ]
机构：
[1] CSIR, REG RES LAB, BIOTECHNOL UNIT, TRIVANDRUM 695019, KERALA, INDIA
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1996年 / 23卷 / 06期
关键词：
D O I：
10.1111/j.1472-765X.1996.tb01346.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glucoamylases produced by Aspergillus niger grown on wheat bran in solid cultures were purified. Four different forms, GA I, GA I', GA II and GA III, were found having apparent molecular weights of 112000, 104000, 74000 and 61000 Da respectively. The enzymes are glycoproteins with a carbohydrate content of 16%, and optimal activity at 60 degrees C and pH 4.4. Activity was strongly inhibited by Hg2+ while Mn2+ and Fe2+ were stimulatory. The K-m values for the degradation of starch and maltose were 3.5 and 7.8 mg ml(-1), respectively.
引用
收藏
页码：403 / 406
页数：4
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