Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation

被引：30
|
作者：
Selvakumar, P [1 ]
Ashakumary, L [1 ]
Helen, A [1 ]
Pandey, A [1 ]
机构：
[1] CSIR, REG RES LAB, BIOTECHNOL UNIT, TRIVANDRUM 695019, KERALA, INDIA
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1996年 / 23卷 / 06期
关键词：
D O I：
10.1111/j.1472-765X.1996.tb01346.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Glucoamylases produced by Aspergillus niger grown on wheat bran in solid cultures were purified. Four different forms, GA I, GA I', GA II and GA III, were found having apparent molecular weights of 112000, 104000, 74000 and 61000 Da respectively. The enzymes are glycoproteins with a carbohydrate content of 16%, and optimal activity at 60 degrees C and pH 4.4. Activity was strongly inhibited by Hg2+ while Mn2+ and Fe2+ were stimulatory. The K-m values for the degradation of starch and maltose were 3.5 and 7.8 mg ml(-1), respectively.
引用
收藏
页码：403 / 406
页数：4
相关论文
共 50 条
  • [1] PURIFICATION AND CHARACTERIZATION OF PHYTASE PRODUCED FROM ASPERGILLUS NIGER USING SOLID STATE FERMENTATION
    Mahmood, Shahzad
    Shahid, Memuna ghafoor
    Nadeem, Muhammad
    Nelofer, Rubina
    Irfan, Muhammad
    CELLULOSE CHEMISTRY AND TECHNOLOGY, 2024, 58 (9-10): : 1091 - 1098
  • [2] Biochemical Characterisation of a Glucoamylase from Aspergillus niger Produced by Solid-State Fermentation
    Slivinski, Christiane Trevisan
    Lopes Machado, Alex Vinicius
    Iulek, Jorge
    Ayub, Ricardo Antnio
    de Almeida, Mareci Mendes
    BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, 2011, 54 (03) : 559 - 568
  • [3] Extraction and purification of amyloglucosidase produced by solid state fermentation with Aspergillus niger
    Ramadas, M
    Holst, O
    Mattiasson, B
    BIOTECHNOLOGY TECHNIQUES, 1995, 9 (12) : 901 - 906
  • [4] PRODUCTION OF GLUCOAMYLASE BY ASPERGILLUS NIGER UNDER SOLID STATE FERMENTATION
    Nahid, P.
    Vossoughi, M.
    Roostaazad, R.
    Ahmadi, M.
    Zarrabi, A.
    Hosseini, S. M.
    INTERNATIONAL JOURNAL OF ENGINEERING, 2012, 25 (01): : 1 - 7
  • [5] Optimization of extraction and purification of glucoamylase produced by Aspergillus awamori in solid-state fermentation
    Sangeeta Negi
    Rintu Banerjee
    Biotechnology and Bioprocess Engineering, 2009, 14 : 60 - 66
  • [6] Optimization of extraction and purification of glucoamylase produced by Aspergillus awamori in solid-state fermentation
    Negi, Sangeeta
    Banerjee, Rintu
    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2009, 14 (01) : 60 - 66
  • [7] Optimization of glucoamylase production by Aspergillus niger in solid-state fermentation
    Silveira, ST
    Oliveira, MS
    Costa, JAV
    Kalil, SJ
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2006, 128 (02) : 131 - 139
  • [8] Solid-state fermentation applications in glucoamylase production by Aspergillus niger
    Ahmadi, Mitra
    CLINICAL BIOCHEMISTRY, 2011, 44 (13) : S240 - S241
  • [9] Optimization of glucoamylase production by Aspergillus niger in solid-state fermentation
    Silvana T. Silveira
    Melissa S. Oliveira
    Jorge A. V. Costa
    Susana J. Kalil
    Applied Biochemistry and Biotechnology, 2006, 128 : 131 - 139
  • [10] GLUCOAMYLASE PRODUCTION FROM ASPERGILLUS NIGER BY USING SOLID STATE FERMENTATION PROCESS
    Imran, Muhammad
    Asad, Muhammad Javaid
    Gulfraz, Muhammad
    Qureshi, Rahmatullah
    Gul, Hina
    Manzoor, Nazish
    Choudhary, Arshad Nawaz
    PAKISTAN JOURNAL OF BOTANY, 2012, 44 (06) : 2103 - 2110
12345