A New Tospovirus sp in Cucurbit Crops in Mexico

被引：28

作者：

Ciuffo, M. ^{[1
]}

Kurowski, C. ^{[2
]}

Vivoda, E. ^{[2
]}

Copes, B. ^{[2
]}

Masenga, V. ^{[2
]}

Falk, B. W. ^{[3
]}

Turina, M. ^{[1
]}

机构：

[1] Ist Virol Vegetale, I-10135 Turin, Italy

[2] Harris Moran Seed Co, Davis, CA 95618 USA

[3] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA

来源：

PLANT DISEASE | 2009年 / 93卷 / 05期

关键词：

VIRUS; TOMATO; IDENTIFICATION; GENE; WATERMELON; CHRYSANTHEMUM; SUPPRESSOR; PROTEIN; SQUASH; FAMILY;

D O I：

10.1094/PDIS-93-5-0467

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

During the 2007 growing season, melon (Cucumis melo) samples from the state of Guerrero in Mexico showing mosaie and other virus-like symptoms were collected for analysis. Electron microscopic examination of negatively stained leaf-dip extracts revealed the presence of abundant virus-like particles with features characteristic of the family Bunyavirdiae. No other viral particles were observed in these preparations. However, enzyme-linked immunosorbent assays (ELISAs) specific for the most common Tospovirus spp. gave negative results. Antibodies raised against purified nucleocapsids reacted specifically with the infected leaf extracts in Western blots and double-antibody sandwich ELISA. The viral RNA was used as a template for a cDNA library, and nucleotide sequence analysis identified cloned cDNAs representing sequences corresponding to the three Tospovirus genome segments. Sequence comparisons showed that the new virus had the highest similarity of Chrysanthemum stem necrosis virus (CSNV). Phylogenetic analysis of two genome regions confirmed that this virus, provisionally named Melon severe mosaie virus (MeSMV), is a previously undescribed Tospovirus sp. belonging to the "new world" clade of Tospovirus spp. An initial survey of various cucurbit crops in various states of Mexico confirmed the widespread occurrence of this virus.

引用

页码：467 / 474

页数：8

共 41 条

[21] Phylogenetic studies of tospoviruses (Family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs [J].