Protection in mice immunized with a heterologous prime-boost regime using DNA and recombinant pseudorabies expressing TgSAG1 against Toxoplasma gondii challenge

被引：28

作者：

Shang, Limin ^{[1
]}

Liu, Quan ^{[1
]}

Liu, Wensen ^{[1
]}

Men, Jingtao ^{[1
]}

Gao, Shengyan ^{[1
]}

Jiang, Li ^{[1
]}

Wang, Ze ^{[1
]}

Zhai, Yujia ^{[1
]}

Jin, Hongtao ^{[1
]}

Lian, Hai ^{[1
]}

Chen, Chen ^{[1
]}

Xia, Zhiping ^{[1
]}

Yuan, Ziguo ^{[2
]}

Zhu, Xing-Quan ^{[2
]}

机构：

[1] Acad Mil Med Sci, Vet Inst, Parasitol Lab, Changchun 130062, Jilin Province, Peoples R China

[2] S China Agr Univ, Coll Vet Med, Dept Parasitol, Guangzhou 510642, Guangdong, Peoples R China

来源：

VACCINE | 2009年 / 27卷 / 21期

关键词：

Toxoplasma gondii; TgSAG1; Pseudorabies virus; DNA vaccine; Prime-boost regime; Mice; VISCERAL LEISHMANIASIS; SURFACE-ANTIGEN; T-CELLS; VACCINE; INFECTION; VIRUS; SAG1; PROTEIN; GENE; STRATEGIES;

D O I：

10.1016/j.vaccine.2009.03.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An effective vaccine of animals call block transmission of Toxoplasma gondii to humans. In this study, mice have been protected against lethal T gondii challenge by a prime-boost vaccination strategy using DNA vaccine pVAX/TgSAG1 and recombinant pseudorabies Virus rPRV/TgSAG1, both expressing the major immunodominant surface antigen of T. gondii (TgSAG1). High levels of splenocyte proliferative responses and significant levels of IFN-gamma resulted, with strong cytotoxic T lymphocyte (CTL) responses in vitro. After lethal challenge, prime-boost vaccinated mice showed all increased survival time (15.4 +/- 5.0 days) and a 40% survival rate compared with controls who all died within 11 days of challenge. Results of the present Study indicated that this novel immunization strategy is useful ill enhancing immune protection in mice against lethal 7: gondii infection, which would provide foundation for the development of effective vaccines against T. gondii. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2741 / 2745

页数：5

共 35 条

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