Transmission Structured Illumination Microscopy for Quantitative Phase and Scattering Imaging

被引：4

作者：

Wen, Kai ^{[1
]}

Ma, Ying ^{[1
]}

Liu, Min ^{[1
]}

Li, Jianlang ^{[1
]}

Zalevsky, Zeev ^{[2
,3
]}

Zheng, Juanjuan ^{[1
]}

机构：

[1] Xidian Univ, Sch Phys & Optoelect Engn, Xian, Peoples R China

[2] Bar Ilan Univ, Fac Engn, Ramat Gan, Israel

[3] Bar Ilan Univ, Nano Technol Ctr, Ramat Gan, Israel

来源：

FRONTIERS IN PHYSICS | 2021年 / 8卷

基金：

中国国家自然科学基金;

关键词：

quantitative phase microscopy; structured illumination; phase gradient; resolution enhancement; multimodality imaging; RESOLUTION ENHANCEMENT; SUPERRESOLUTION; CONTRAST; LIGHT; INTERFEROMETRY; RETRIEVAL;

D O I：

10.3389/fphy.2020.630350

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

In this paper, we demonstrate a digital micromirror device (DMD) based optical microscopic apparatus for quantitative differential phase contrast (qDIC) imaging, coherent structured illumination microscopy (SIM), and dual-modality (scattering/fluorescent) imaging. For both the qDIC imaging and the coherent SIM, two sets of fringe patterns with orthogonal orientations and five phase-shifts for each orientation, are generated by a DMD and projected on a sample. A CCD camera records the generated images in a defocusing manner for qDIC and an in-focus manner for coherent SIM. Both quantitative phase images and super-resolved scattering/fluorescence images can be reconstructed from the recorded intensity images. Moreover, fluorescent imaging modality is integrated, providing specific biochemical structures of the sample once using fluorescent labeling.

引用

页数：7

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