Nanobioconjugates of Candida antarctica lipase B and single-walled carbon nanotubes in biodiesel production

被引:56
作者
Bencze, Laszlo Csaba [1 ]
Bartha-Vari, Judith H. [1 ]
Katona, Gabriel [1 ]
Tosa, Monica Ioana [1 ]
Paizs, Csaba [1 ]
Irimie, Florin-Dan [1 ]
机构
[1] Babes Bolyai Univ Cluj Napoca, Biocatalysis & Biotransformat Res Grp, RO-400028 Cluj Napoca, Romania
关键词
Lipase B from Candida antarctica; Single-walled carbon nanotubes; Covalent immobilization; Biodiesel production; BURKHOLDERIA-CEPACIA LIPASE; IMMOBILIZATION; TRANSESTERIFICATION; MODULATION; METHANOL; BATCH;
D O I
10.1016/j.biortech.2015.10.072
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Carboxylated single-walled carbon nanotubes (SWCNTCOOH) were used as support for covalent immobilization of Candida antarctica lipase B (CaL-B) using linkers with different lengths. The obtained nanostructured biocatalysts with low diffusional limitation were tested in batch mode in the ethanolysis of the sunflower oil. SWCNTCOOH-CaL-B proved to be a highly efficient and stable biocatalyst in acetonitrile (83.4% conversion after 4 h at 35 degrees C, retaining > 90% of original activity after 10 cycles). (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:853 / 860
页数:8
相关论文
共 35 条
[1]  
Adriana G, 2011, STUD U BABES-BOL CHE, V56, P71
[2]  
AOAC Method, 2005, OFFICIAL METHODS ANA, P41
[3]   Modulation of the properties of immobilized CALB by chemical modification with 2,3,4-trinitrobenzenesulfonate or ethylendiamine. Advantages of using adsorbed lipases on hydrophobic supports [J].
Barbosa, Oveimar ;
Ruiz, Monica ;
Ortiz, Claudia ;
Fernandez, Marisol ;
Torres, Rodrigo ;
Fernandez-Lafuente, Roberto .
PROCESS BIOCHEMISTRY, 2012, 47 (05) :867-876
[4]   Immobilization of Phenylalanine Ammonia-Lyase on Single-Walled Carbon Nanotubes for Stereoselective Biotransformations in Batch and Continuous-Flow Modes [J].
Bartha-Vari, Judith H. ;
Tosa, Monica I. ;
Irimie, Florin-Dan ;
Weiser, Diana ;
Boros, Zoltan ;
Vertessy, Beata G. ;
Paizs, Csaba ;
Poppe, Laszlo .
CHEMCATCHEM, 2015, 7 (07) :1122-1128
[5]   The influence of carbon nanotubes on enzyme activity and structure: investigation of different immobilization procedures through enzyme kinetics and circular dichroism studies [J].
Cang-Rong, Jason Teng ;
Pastorin, Giorgia .
NANOTECHNOLOGY, 2009, 20 (25)
[6]   Magnetic Cross-Linked Enzyme Aggregates (mCLEAs) of Candida antarctica Lipase: An Efficient and Stable Biocatalyst for Biodiesel Synthesis [J].
Cruz-Izquierdo, Alvaro ;
Pico, Enrique A. ;
Lopez, Carmen ;
Serra, Juan L. ;
Llama, Maria J. .
PLOS ONE, 2014, 9 (12)
[7]   Comparison of transesterification methods for production of biodiesel from vegetable oils and fats [J].
Demirbas, Ayhan .
ENERGY CONVERSION AND MANAGEMENT, 2008, 49 (01) :125-130
[8]   Biodiesel production using enzymatic transesterification - Current state and perspectives [J].
Gog, Adriana ;
Roman, Marius ;
Tosa, Monica ;
Paizs, Csaba ;
Irimie, Florin Dan .
RENEWABLE ENERGY, 2012, 39 (01) :10-16
[9]   Advances in synthesis of biodiesel via enzyme catalysis: Novel and sustainable approaches [J].
Guldhe, Abhishek ;
Singh, Bhaskar ;
Mutanda, Taurai ;
Perrnaul, Kugen ;
Bux, Faizal .
RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2015, 41 :1447-1464
[10]   Activity Enhancement of Candida antarctica Lipase B by Flexibility Modulation in Helix Region Surrounding the Active Site [J].
Hong, So Yeon ;
Yoo, Young Je .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2013, 170 (04) :925-933