Adaptation of bioassay to detect endothelium-derived relaxing factors from the canine atrial endocardium

被引:2
作者
Chua, Yeow Leng [1 ]
Barbosa Evora, Paulo Roberto [2 ]
Celotto, Andrea Carla
Pearson, Paul Joseph
Discigil, Berent [3 ]
Schaff, Hartzell Vernon [4 ]
机构
[1] Natl Heart Ctr Singapore, Dept Cardiothorac Surg, Singapore, Singapore
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Surg & Anat Head, BR-14049 Ribeirao Preto, SP, Brazil
[3] Adnan Menderes Univ, Fac Med, Dept Cardiovasc Surg, Aydin, Turkey
[4] Mayo Clin, Rochester, MN USA
来源
REVISTA BRASILEIRA DE CIRURGIA CARDIOVASCULAR | 2009年 / 24卷 / 02期
关键词
Heart atria; Endothelium; Nitric oxide; Endocardium; NITRIC-OXIDE; DEPENDENT RELAXATION; VASCULAR ENDOTHELIUM; PLATELET-AGGREGATION; CARDIAC ENDOTHELIUM; CORONARY-ARTERIES; SMOOTH-MUSCLE; FACTOR EDRF; CROSS-TALK; PROSTACYCLIN;
D O I
10.1590/S0102-76382009000200020
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: The aim of this study was to assess the release of endothelium-derived relaxing factors from the endocardium of canine atrial appendage. Methods: To study the release of endothelium-derived relaxing factor (EDRF) from intact atrial endocardial endothelium, tube-shaped sutures of canine atrial appendages were performed and effluents from these tubes were bioassayed (isolated perfused organ chamber system) for detection of EDRF in canine coronary artery. Results: Effluent from the right atrial appendage caused a relaxation of 58.4 +/- 10.1% and the left atrial appendage 74.9 +/- 8.5% from the initial prostagladin F2 alpha contraction in coronary artery. No significant statistical difference was detected in effluent from the right and left atrial appendages. This relaxation was abolished by treating the heart tubes with Triton X-100 and reduced by treatment with LNMMA, a competitive inhibitor of nitric oxide and with indomethacin, an inhibitor of the cyclo-oxygenase pathway, also indicating the release of vasodilatory prostanoids from the endocardial endothelium. Conclusion: This study showed for the first time, in vitro luminal release of EDRF and prostacyclin from the canine heart atrium. The ability of the endocardial endothelium to produce these factors could play an important role in preventing thrombus formation in the cardiac chambers.
引用
收藏
页码:225 / 232
页数:8
相关论文
共 44 条
[1]   HUMAN-ENDOTHELIAL CELLS INHIBIT PLATELET-AGGREGATION BY SEPARATELY STIMULATING PLATELET CYCLIC-AMP AND CYCLIC-GMP [J].
ALHEID, U ;
REICHWEHR, I ;
FORSTERMANN, U .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1989, 164 (01) :103-110
[2]   Vascular System: Role of Nitric Oxide in Cardiovascular Diseases [J].
Bian, Ka ;
Doursout, Marie-Francoise ;
Murad, Ferid .
JOURNAL OF CLINICAL HYPERTENSION, 2008, 10 (04) :304-310
[3]   Cardiac endothelium and myocardial function [J].
Brutsaert, DL ;
Fransen, P ;
Andries, LJ ;
De Keulenaer, GW ;
Sys, SU .
CARDIOVASCULAR RESEARCH, 1998, 38 (02) :281-290
[4]   Cardiac endothelial-myocardial signaling: Its role in cardiac growth, contractile performance, and rhythmicity [J].
Brutsaert, DL .
PHYSIOLOGICAL REVIEWS, 2003, 83 (01) :59-115
[5]   The cardiac endothelium: Functional morphology, development, and physiology [J].
Brutsaert, DL ;
DeKeulenaer, GW ;
Fransen, P ;
Mohan, P ;
Kaluza, GL ;
Andries, LJ ;
Rouleau, JL ;
Sys, SU .
PROGRESS IN CARDIOVASCULAR DISEASES, 1996, 39 (03) :239-262
[6]   The inducible nitric-oxide synthase modulates endothelin-1-dependent release of prostacyclin and inhibition of platelet aggregation ex vivo in the mouse [J].
Carrier, Emilie ;
Brochu, Isabelle ;
de Brum-Fernandes, Artur J. ;
D'Orleans-Juste, Pedro .
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2007, 323 (03) :972-978
[7]  
CHUA YL, 1993, CIRCULATION, V88, P128
[8]   RELEASE AND PROPERTIES OF ENDOTHELIUM-DERIVED RELAXING FACTOR (EDRF) FROM ENDOTHELIAL-CELLS IN CULTURE [J].
COCKS, TM ;
ANGUS, JA ;
CAMPBELL, JH ;
CAMPBELL, GR .
JOURNAL OF CELLULAR PHYSIOLOGY, 1985, 123 (03) :310-320
[9]   NOVEL TECHNIQUE TO BIOASSAY ENDOCARDIUM-DERIVED NITRIC-OXIDE FROM THE BEATING HEART [J].
DISCIGIL, B ;
PEARSON, PJ ;
CHUA, YL ;
EVORA, PRB ;
SECCOMBE, JF ;
SCHAFF, HV .
ANNALS OF THORACIC SURGERY, 1995, 59 (05) :1182-1186
[10]   ENDOTHELIAL-CELLS AS MEDIATORS OF VASODILATION OF ARTERIES [J].
FURCHGOTT, RF ;
CHERRY, PD ;
ZAWADZKI, JV ;
JOTHIANANDAN, D .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1984, 6 :S336-S343