High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic-electrospray ionisation mass spectrometric analysis of selected alkaloid groups

被引:56
作者
Stöckigt, J
Sheludko, Y
Unger, M
Gerasimenko, I
Warzecha, H
Stöckigt, D
机构
[1] Johannes Gutenberg Univ Mainz, Inst Pharm, Dept Pharmaceut Biol, D-55099 Mainz, Germany
[2] Natl Acad Sci Ukraine, Inst Cell Biol & Genet Engn, UA-252143 Kiev, Ukraine
[3] AnalytiCon AG, D-56626 Andernach, Germany
关键词
reviews; hybrid plant cell suspension cultures; Rauvolfia serpentina; Rhazya stricta; Aspidosperma quebracho-blanco; Apocynaceae; monoterpenoid indole alkaloids; alkaloids; beta-carboline alkaloids;
D O I
10.1016/S0021-9673(02)00037-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Systems for efficient separation of selected alkaloid groups by high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and capillary electrophoresis coupled with electrospray ionisation mass spectrometry (CE-ESI-MS) are described. The optimized HPLC system was applied for the separation of 23 standard indole alkaloids as well as for qualitative and quantitative analyses of crude alkaloid extracts of Rauvolfia serpentinaXRhazya stricta hybrid cell cultures. The developed conditions for CE analysis proved to be efficient for separation of mixtures of standard indole and beta-carboline alkaloids. The described buffer system is also applicable in the combination of CE with electrospray ionisation mass spectrometry. This analytical technique allowed the separation and identification of components of standard indole alkaloid mixture as well as crude extracts of R. serpentina roots, R. serpentina cell suspension cultures and cortex of Aspidosperma quebracho-blanco. The influence of buffer composition and analyte structures on separation is discussed. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:85 / 113
页数:29
相关论文
共 129 条
[1]   METHYL JASMONATE VAPOR INCREASES THE DEVELOPMENTALLY CONTROLLED SYNTHESIS OF ALKALOIDS IN CATHARANTHUS AND CINCHONA SEEDLINGS [J].
AERTS, RJ ;
GISI, D ;
DECAROLIS, E ;
DELUCA, V ;
BAUMANN, TW .
PLANT JOURNAL, 1994, 5 (05) :635-643
[2]  
Aimi N, 1996, CHEM PHARM BULL, V44, P1637, DOI 10.1248/cpb.44.1637
[3]   LEAF ALKALOIDS OF RAUWOLFIA-VOMITORIA [J].
AMER, MM ;
COURT, WE .
PHYTOCHEMISTRY, 1980, 19 (08) :1833-1836
[4]  
AMER MMA, 1980, PLANTA MED, P8
[5]   SEPARATION OF 8 ALKALOIDS AND MECONIC ACID AND QUANTITATION OF 5 PRINCIPAL ALKALOIDS IN GUM OPIUM BY GRADIENT REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
AYYANGAR, NR ;
BHIDE, SR .
JOURNAL OF CHROMATOGRAPHY, 1988, 436 (03) :455-465
[6]  
Bailey G, 1988, ELECTROSTATIC SPRAYI
[7]   THE RESERPINE, RESCINNAMINE, AND DESERPIDINE CONTENT OF RAUWOLFIA ROOTS [J].
BANES, D ;
HOUK, AEH ;
WOLFF, J .
JOURNAL OF THE AMERICAN PHARMACEUTICAL ASSOCIATION, 1958, 47 (09) :625-627
[8]   Analysis of basic pharmaceuticals by capillary electrophoresis in coated capillaries and on-line mass spectrometric detection [J].
Belder, D ;
Stockigt, D .
JOURNAL OF CHROMATOGRAPHY A, 1996, 752 (1-2) :271-277
[9]   Nonaqueous capillary electrophoresis - its applicability in the analysis of food, pharmaceuticals and biological fluids [J].
Bjornsdottir, I ;
Tjornelund, J ;
Hansen, SH .
ELECTROPHORESIS, 1998, 19 (12) :2179-2186
[10]   DETERMINATION OF OPIUM-ALKALOIDS IN OPIUM BY CAPILLARY ELECTROPHORESIS [J].
BJORNSDOTTIR, I ;
HANSEN, SH .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1995, 13 (4-5) :687-693