The proteome of Mannheimia succiniciproducens, a capnophilic rumen bacterium

被引：40

作者：

Lee, Jeong Wook

Lee, Sang Yup

Song, Hyohak

Yoo, Jong-Shin

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Metab & Biomol Engn Natl Res Lab, BioProc Engn Res Ctr, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Bioinformat Res Ctr, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Dept BioSyst, Taejon 305701, South Korea

[4] Korea Basic Sci Inst, Taejon 305701, South Korea

来源：

PROTEOMICS | 2006年 / 6卷 / 12期

关键词：

genome; proteome; Mannheimia succiniciproducens; rumen bacterium;

D O I：

10.1002/pmic.200500837

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is an industrially important bacterium as an efficient succinic acid producer. Recently, its full genome sequence was determined. In the present study, we analyzed the M. succiniciproducens proteome based on the genome information using 2-DE and MS. We established proteome reference map of M. succiniciproducens by analyzing whole cellular proteins, membrane proteins, and secreted proteins. More than 200 proteins were identified and characterized by MS/MS supported by various bioinformatic tools. The presence of proteins previously annotated as hypothetical proteins or proteins having putative functions were also confirmed. Based on the proteome reference map, cells in the different growth phases were analyzed at the proteome level. Comparative proteome profiling revealed valuable information to understand physiological changes during growth, and subsequently suggested target genes to be manipulated for the strain improvement.

引用

页码：3550 / 3566

页数：17

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