Fluorescence polarization-based detection of cancer-related mutations using target-initiated rolling circle amplification

被引：10

作者：

Kwon, Woo Young ^{[1
]}

Cha, Byung Seok ^{[1
]}

Kim, Seokjoon ^{[1
]}

Hwang, Sung Hyun ^{[1
]}

Kim, Ji Min ^{[1
]}

Kalimuthu, Kalishwaralal ^{[1
]}

Park, Hyun Gyu ^{[2
]}

Park, Ki Soo ^{[1
]}

机构：

[1] Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea

[2] Korea Adv Inst Sci & Technol, BK 21 Program, Dept Chem & Biomol Engn, Daehak Ro 291, Daejeon 34141, South Korea

来源：

ANALYST | 2019年 / 144卷 / 14期

基金：

新加坡国家研究基金会;

关键词：

BIOMARKERS;

D O I：

10.1039/c9an00429g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We devised a new method to detect cancer-related mutations based on target-initiated rolling circle amplification in combination with fluorescence polarization. We then applied this method to identify the presence of KRAS G13D and G12D, two of the most frequent mutations found in colorectal cancer patients, demonstrating high sensitivity and specificity.

引用

页码：4149 / 4152

页数：4

共 23 条

[1]

[Anonymous], BIOTECHNOL J

[2]

[Anonymous], MEDSCAPE

[3]

[Anonymous], CANCER

[4]

[Anonymous], SENS ACTUATORS B