A fiber-optic DNA biosensor microarray for the analysis of gene expression

被引：184

作者：

Ferguson, JA

Boles, TC

Adams, CP

Walt, DR

机构：

[1] TUFTS UNIV,DEPT CHEM,MAX TISHLER LAB ORGAN CHEM,MEDFORD,MA 02155

[2] MOSAIC TECHNOL,BOSTON,MA 02115

来源：

NATURE BIOTECHNOLOGY | 1996年 / 14卷 / 13期

关键词：

array sensors; oligonucleotides; fiber optics; fluorescence;

D O I：

10.1038/nbt1296-1681

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A fiber-optic biosensor array is described for the simultaneous analysis of multiple DNA sequences. A bundle of optical fibers was assembled with each fiber carrying a different oligonucleotide probe immobilized on its distal end. Hybridization of fluorescently labeled complementary oligonucleotides to the array was monitored by observing the increase in fluorescence that accompanied binding. The approach enables fast (<10 min) and sensitive (10 nM) detection of multiple DNA sequences simultaneously, with the potential for quantitative hybridization analysis.

引用

页码：1681 / 1684

页数：4

共 41 条

[1]

BEATTIE KL, 1995, MOL PATHOLOGY, V41, P700

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