Applications of asymmetrical flow field-flow fractionation for separation and characterization of polysaccharides: A review

被引：24

作者：

Chen, Xue ^{[1
]}

Zhang, Wenhui ^{[3
]}

Dou, Yuwei ^{[1
]}

Song, Tiange ^{[3
]}

Shen, Shigang ^{[3
]}

Dou, Haiyang ^{[1
,2
]}

机构：

[1] Hebei Univ, Sch Basic Med Sci, Key Lab Pathogenesis Mech & Control Inflammatory, Baoding 071000, Peoples R China

[2] Hebei Univ, Affiliated Hosp, Baoding 071000, Peoples R China

[3] Hebei Univ, Coll Chem & Environm Sci, Key Lab Analyt Sci & Technol Hebei Prov, Baoding 071002, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2021年 / 1635卷

关键词：

Polysaccharide; Separation; Characterization; Asymmetrical flow field-flow fractionation; CEREAL BETA-GLUCAN; MOLAR-MASS; MOLECULAR CHARACTERIZATION; PARTICLE-SIZE; BRANCHED POLYSACCHARIDES; IONIC-STRENGTH; BARLEY MALT; STARCH; MACROMOLECULES; NANOPARTICLES;

D O I：

10.1016/j.chroma.2020.461726

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polysaccharides are the most abundant natural biopolymers on the earth and are widely used in food, medicine, materials, cosmetics, and other fields. The physicochemical properties of polysaccharides such as particle size and molecular weight often affect their practical applications. In recent years, asymmetrical flow field-flow fractionation (AF4) has been widely used in the separation and characterization of polysaccharides because it has no stationary phases or packing materials, which reduces the risk of shear degradation of polysaccharides. In this review, the principle of AF4 was introduced briefly. The operation conditions of AF4 for the analysis of polysaccharides were discussed. The applications of AF4 for the separation and characterization of polysaccharides from different sources (plants, animals, and microorganisms) over the last decade were critically reviewed. (C) 2020 Elsevier B.V. All rights reserved.

引用

页数：9

共 83 条

[1] Characterization of sodium hyaluronate blends using frit inlet asymmetrical flow field-flow fractionation and multiangle light scattering [J].