Essential genes are more evolutionarily conserved than are nonessential genes in bacteria

被引：431

作者：

Jordan, IK ^{[1
]}

Rogozin, IB ^{[1
]}

Wolf, YI ^{[1
]}

Koonin, EV ^{[1
]}

机构：

[1] Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA

来源：

GENOME RESEARCH | 2002年 / 12卷 / 06期

关键词：

D O I：

10.1101/gr.87702

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The "knockout-rate" prediction holds that essential genes should be more evolutionarily conserved than are nonessential genes. This is because negative (purifying) selection acting on essential genes is expected to be more stringent than that for nonessential genes, which are more functionally dispensable and/or redundant. However, a recent survey of evolutionary distances between Saccharomyces cerevisiae and Caenorhabditis elegans proteins did not reveal any difference between the rates of evolution for essential and nonessential genes. An analysis of mouse and rat orthologous genes also found that essential and nonessential genes evolved at similar rates when genes thought to evolve under directional selection were excluded from the analysis. In the present study, we combine genomic Sequence data with experimental knockout data to compare the rates of evolution and the levels of selection for essential versus nonessential bacterial genes. In contrast to the results obtained for eukaryotic genes, essential bacterial genes appear to be more conserved than are nonessential genes over both relatively short (microevolutionary) and longer (macroevolutionary) time scales.

引用

页码：962 / 968

页数：7

共 30 条

[1] Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori [J].

Alm, RA ;

Ling, LSL ;

Moir, DT ;

King, BL ;

Brown, ED ;

Doig, PC ;

Smith, DR ;

Noonan, B ;

Guild, BC ;

deJonge, BL ;

Carmel, G ;

Tummino, PJ ;

Caruso, A ;

Uria-Nickelsen, M ;

Mills, DM ;

Ives, C ;

Gibson, R ;

Merberg, D ;

Mills, SD ;

Jiang, Q ;

Taylor, DE ;

Vovis, GF ;

Trost, TJ .

NATURE, 1999, 397 (6715) :176-180

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[4]

[Anonymous], INTELL SYST MOL BIOL

[5] The complete genome sequence of Escherichia coli K-12 [J].

Blattner, FR ;

Plunkett, G ;

Bloch, CA ;

Perna, NT ;

Burland, V ;

Riley, M ;

ColladoVides, J ;

Glasner, JD ;

Rode, CK ;

Mayhew, GF ;

Gregor, J ;

Davis, NW ;

Kirkpatrick, HA ;

Goeden, MA ;

Rose, DJ ;

Mau, B ;

Shao, Y .

SCIENCE, 1997, 277 (5331) :1453-+

[6] Evolution: What determines the rate of sequence evolution? [J].

Brookfield, JFY .

CURRENT BIOLOGY, 2000, 10 (11) :R410-R411

[7] TN5TAC1, A DERIVATIVE OF TRANSPOSON TN5 THAT GENERATES CONDITIONAL MUTATIONS [J].

CHOW, WY ;

BERG, DE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6468-6472

[8]

GRISHIN NV, 1995, J MOL EVOL, V41, P675

[9] MOLECULAR ANALYSIS OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-I - ON THE NUMBER OF GENES AND THE IDENTIFICATION OF ESSENTIAL GENES USING TEMPERATURE-SENSITIVE-LETHAL MUTATIONS [J].

HARRIS, SD ;

CHENG, J ;

PUGH, TA ;

PRINGLE, JR .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 225 (01) :53-65

[10] Protein dispensability and rate of evolution [J].

Hirsh, AE ;

Fraser, HB .

NATURE, 2001, 411 (6841) :1046-1049

← 1 2 3 →