MicroRNA-486-5p targeting PIM-1 suppresses cell proliferation in breast cancer cells

被引:88
作者
Zhang, Guoqiang [1 ]
Liu, Zengyan [2 ]
Cui, Guanghe [3 ]
Wang, Xiaohong [1 ]
Yang, Zhenlin [1 ]
机构
[1] Binzhou Med Coll, Affiliated Hosp, Dept Thyroid & Breast Surg, Binzhou 256603, Peoples R China
[2] Binzhou Med Coll, Affiliated Hosp, Dept Hematol, Binzhou 256603, Peoples R China
[3] Binzhou Med Coll, Affiliated Hosp, Dept Ultrason Med, Binzhou 256603, Peoples R China
基金
中国国家自然科学基金;
关键词
miR-486-5p; PIM-1; Breast cancer; Proliferation; DIFFERENTIAL EXPRESSION; TUMOR PROGRESSION; PROSTATE-CANCER; KINASE PIM-1; LUNG-CANCER; METASTASIS; BIOMARKERS; TRANSCRIPTOME; INSTABILITY; CARCINOMA;
D O I
10.1007/s13277-014-2412-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
MicroRNAs (miRNAs) are emerging as critical regulators in carcinogenesis and tumor progression. Recently, miR-486-5p has been proved to play an important role in several cancers, but its functions in the context of breast cancer (BC) remain unknown. In this study, we found that miR-486-5p expression is significantly downregulated in BC tissues and cell lines. Overexpression of miR-486-5p dramatically suppressed BC cell proliferation in vitro and in vivo, induced G0/G1 arrest, and promoted apoptosis. We subsequently identified the oncogene PIM-1 as a direct target of miR-486-5p in BC. Overexpression of PIM-1 attenuated the function of miR-486-5p in BC cells. Together, we conclude that miR-486-5p exerts its antiproliferative function by directly downregulating PIM-1 expression. This novel miR-486-5p/PIM-1 axis provides insight into the pathogenesis of BC and might be therapeutic targets for prevention or treatment of BC.
引用
收藏
页码:11137 / 11145
页数:9
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