Homologous recombination between different species of alphaherpesviruses has been described between herpes simplex viruses 1 and 2 but has not yet been observed between other alphaherpesviruses. In the present study we chose to assess to what extent in vitro recombination can occur between members of a well-defined group of closely related viruses such as ruminant alphaherpesviruses. At 24 h after infection of epithelial bovine kidney cells with a double-deleted mutant of bovine herpesvirus 1 (BoHV-1) (containing green fluorescent protein and red fluorescent protein genes) and different ruminant alphaherpesviruses, four types of progeny viruses were detected and distinguished according to their phenotype. Frequent recombination events between identical or different strains of BoHV-1 were observed (up to 30%), whereas only two BoHV-1/BoHV-5 recombinants were identified, and no recombinants between BoHV-1 and less closely related caprine and cervine herpesviruses were detected. Restriction analysis of the genomes of the two BoHV-1/BoHV-5 recombinants showed different genetic backgrounds. One possessed a restriction pattern close to BoHV-1, whereas the other one was close to BoHV-5. This exhaustive analysis of each combination of coinfection in a unique situation of five closely related alphaherpesviruses revealed the importance of a high degree of genetic relatedness and similar parental virus growth kinetics for successful interspecific recombination.
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Univ Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, JapanUniv Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
Kohmoto, Tomohiro
Naruto, Takuya
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Univ Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, JapanUniv Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
Naruto, Takuya
Watanabe, Miki
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Univ Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, JapanUniv Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
Watanabe, Miki
Fujita, Yuji
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Univ Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, JapanUniv Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
Fujita, Yuji
Ujiro, Sae
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Univ Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, JapanUniv Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
Ujiro, Sae
Okamoto, Nana
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Kobe Univ, Grad Sch Med, Dept Oral & Maxillofacial Surg, Kobe, Hyogo, JapanUniv Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
Okamoto, Nana
Horikawa, Hideaki
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Univ Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
Univ Tokushima, Grad Sch Biomed Sci, Support Ctr Adv Med Sci, Tokushima, JapanUniv Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan
Horikawa, Hideaki
Masuda, Kiyoshi
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Univ Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, JapanUniv Tokushima, Grad Sch Biomed Sci, Dept Human Genet, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan